RNA Conformation in Catalytically Active Human Telomerase
| Autor: | Justin A. Yeoman, Beth Ashbridge, Angel Orte, Shankar Balasubramanian, David Klenerman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Telomerase
Base Sequence Chemistry RNA Conformation Molecular Sequence Data RNA RNA-dependent RNA polymerase General Chemistry Biochemistry Molecular biology Catalysis Article Cell biology Enzyme Activation Telomerase RNA component Enzyme activator Colloid and Surface Chemistry Fluorescence microscope Biocatalysis Humans Nucleic Acid Conformation Telomerase reverse transcriptase |
| Popis: | We have used single-molecule fluorescence microscopy to study the folded state of human telomerase RNA (hTR). Here we show that hTR adopts a new conformation on binding to human telomerase reverse transcriptase (hTERT) and reconstitution of an active ribonucleoprotein complex. Our data are consistent with the formation of an RNA pseudoknot in active human telomerase. |
| Databáze: | OpenAIRE |
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