Transcription profile of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with epitheliotropic cutaneous lymphoma
| Autor: | Masahiko Nagata, Kaori Ide, Aki Iio, Nobuo Murayama, Koji Nishifuji, Eiji Ozaki, Yusuke Ito, Naoki Chimura, Takashi Mori, Hiroaki Kamishina, Sadatoshi Maeda |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.medical_treatment C-C chemokine receptor type 7 CXCR3 Chemokine receptor Dogs Mycosis Fungoides medicine Biomarkers Tumor Cytotoxic T cell Animals Dog Diseases Mycosis fungoides General Veterinary business.industry medicine.disease Granzyme B Gene Expression Regulation Neoplastic Cytokine Case-Control Studies Immunology Cytokines Receptors Chemokine business Transcriptome CD8 |
| Zdroj: | Veterinary dermatology. 24(6) |
| ISSN: | 1365-3164 |
| Popis: | Background Mycosis fungoides (MF) is the most common form of canine epitheliotropic cutaneous lymphoma, which is characterized by the accumulation of neoplastic CD8(+) T cells. Given that multifocal skin lesions are commonly seen in MF, neoplastic lymphocytes may actively migrate into the blood circulation. Hypothesis/objectives Cytotoxic T cells with a skin-homing phenotype could be increased in the blood circulation of dogs with MF. Animals Ten dogs with MF and 10 age-matched healthy dogs were included. Methods The transcription levels of chemokine receptors, cytokines and cytotoxic markers in peripheral blood of dogs with MF were quantified by real-time RT-PCR. Results The dogs with MF had lower transcription levels of chemokine receptors associated with skin homing (CCR4), epitheliotropism (CXCR3), lymph node homing (CCR7), a type-1 cytokine (LT-α) and cytotoxic markers (perforin and granzyme B) in the circulation than healthy control dogs (P Conclusions and clinical importance The present results suggest that the number of peripheral cytotoxic T cells with a skin-homing phenotype could be decreased in the peripheral blood of dogs with MF, which might be due to the sequestration of cytotoxic T cells in the lesional skin. |
| Databáze: | OpenAIRE |
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