Melatonin modulate the expression of α1- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress
| Autor: | Slađana Dronjak, Natasa Spasojevic, Bojana Stefanovic, Predrag Jovanovic, Harisa Ferizovic |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Economics and Econometrics medicine.medical_specialty hippocampus α-adrenoceptors Hippocampus Gene Expression melatonin Receptor subtype Melatonin 03 medical and health sciences Mild stress Receptors Adrenergic alpha-2 Internal medicine Receptors Adrenergic alpha-1 Gene expression Materials Chemistry Media Technology medicine Animals Chronic stress RNA Messenger chronic stress Messenger RNA business.industry Forestry β-adrenoceptors Rats 030104 developmental biology Endocrinology Chronic Disease β2 adrenoceptor Receptors Adrenergic beta-2 Receptors Adrenergic beta-1 business Arousal catecholamines Stress Psychological medicine.drug Signal Transduction |
| Zdroj: | Bratislava Medical Journal |
| Popis: | Objective This study investigated the effects of chronic melatonin treatment on gene expression of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus of rats subjected to chronic unpredictable mild stress (CUMS). Background Preclinical studies have also shown that melatonin prevented short- and long-term memory impairments and exhibited antidepressant-like actions. Methods For this study, we used 24 animals, which were divided into four groups, and the experiment lasted 4 weeks. We quantified the changes in mRNA and protein levels of α1-, α2-, β1- and β2-adrenoceptors in the hippocampus after melatonin treatment. Results Our results demonstrated a decreased gene expression of α1-, α2- and β2-adrenoceptors in the hippocampus of rats subjected to unpredictable chronic mild stress, while there was no change in gene expression of β1-adrenoceptors. Melatonin treatment in the CUMS rats prevented the stress-induced decrease in mRNA and protein levels of α1-and β2-adrenoceptors, whereas did not affect either on mRNA or protein level of β1-and α2-adrenoceptors. Conclusion Our data suggest that melatonin, by increasing reduced levels of α1- and β2-adrenoceptors mRNA and protein in the hippocampus of chronic stressed rats, may be beneficial in conditions such as chronic stress and provides an experimental opportunity to probe into further molecular mechanisms underlying the regulation of these receptor subtype (Fig. 2, Ref. 28). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|