Treatment with Isorhamnetin Protects the Brain Against Ischemic Injury in Mice
| Autor: | Jin-Jing Zhao, Shu-Yi Pan, Jin-Qing Song, Kai Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male Inflammation Pharmacology Occludin medicine.disease_cause Biochemistry Neuroprotection Cerebral edema Brain Ischemia Brain ischemia 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Mice 0302 clinical medicine Edema medicine Animals Isorhamnetin Mice Inbred ICR business.industry Brain General Medicine medicine.disease Oxidative Stress 030104 developmental biology Neuroprotective Agents Treatment Outcome chemistry Anesthesia Quercetin medicine.symptom business 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Neurochemical research. 41(8) |
| ISSN: | 1573-6903 |
| Popis: | Ischemic stroke is a major cause of morbidity and mortality, yet lacks effective neuroprotective treatments. The aim of this work was to investigate whether treatment with isorhamnetin protected the brain against ischemic injury in mice. Experimental stroke mice underwent the filament model of middle cerebral artery occlusion with reperfusion. Treatment with isorhamnetin or vehicle was initiated immediately at the onset of reperfusion. It was found that treatment of experimental stroke mice with isorhamnetin reduced infarct volume and caspase-3 activity (a biomarker of apoptosis), and improved neurological function recovery. Treatment of experimental stroke mice with isorhamnetin attenuated cerebral edema, improved blood-brain barrier function, and upregulated gene expression of tight junction proteins including occludin, ZO-1, and claudin-5. Treatment of experimental stroke mice with isorhamnetin activated Nrf2/HO-1, suppressed iNOS/NO, and led to reduced formation of MDA and 3-NT in ipsilateral cortex. In addition, treatment of experimental stroke mice with isorhamnetin suppressed activity of MPO (a biomarker of neutrophil infiltration) and reduced protein levels of IL-1β, IL-6, and TNF-α in ipsilateral cortex. Furthermore, it was found that treatment of experimental stroke mice with isorhamnetin reduced mRNA and protein expression of NMDA receptor subunit NR1 in ipsilateral cortex. In conclusion, treatment with isorhamnetin protected the brain against ischemic injury in mice. Isorhamnetin could thus be envisaged as a countermeasure for ischemic stroke but remains to be tested in humans. |
| Databáze: | OpenAIRE |
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