Fine processes of Nestin-GFP-positive radial glia-like stem cells in the adult dentate gyrus ensheathe local synapses and vasculature
| Autor: | Elias Gebara, Ruadhan O’Laoi, Jacqueline Kocher-Braissant, Nicolas Toni, Irene Sánchez-Pascual, Imane El M’Ghari, Eric A. Bushong, Mark H. Ellisman, Jonathan Moss |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Animals Astrocytes/cytology Cells Cultured Cerebral Arteries/cytology Cerebral Arteries/metabolism Dentate Gyrus/cytology Dentate Gyrus/metabolism Green Fluorescent Proteins Mice Mice Transgenic Nestin/metabolism Neural Stem Cells/cytology Neural Stem Cells/metabolism Neurogenesis/physiology Neuroglia/cytology Neuroglia/metabolism Neurovascular Coupling/physiology Stem Cell Niche/physiology Synapses/metabolism Synapses/ultrastructure Tissue Distribution hippocampus Regenerative Medicine Transgenic Subgranular zone Nestin 0302 clinical medicine Neural Stem Cells Stem Cell Niche Cultured Multidisciplinary Neurogenesis neurogenic niche Neural stem cell adult neurogenesis medicine.anatomical_structure PNAS Plus Neurological Neuroglia Stem Cell Research - Nonembryonic - Non-Human Stem cell Cells 1.1 Normal biological development and functioning Biology adult neural stem cell 03 medical and health sciences Underpinning research medicine electron microscopy Dentate gyrus Neurosciences Cerebral Arteries Stem Cell Research Granule cell 030104 developmental biology nervous system Astrocytes Dentate Gyrus Synapses Neurovascular Coupling Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 18, pp. E2536-45 Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, vol 113, iss 18 |
| ISSN: | 1091-6490 |
| Popis: | Adult hippocampal neurogenesis relies on the activation of neural stem cells in the dentate gyrus, their division, and differentiation of their progeny into mature granule neurons. The complex morphology of radial glia-like (RGL) stem cells suggests that these cells establish numerous contacts with the cellular components of the neurogenic niche that may play a crucial role in the regulation of RGL stem cell activity. However, the morphology of RGL stem cells remains poorly described. Here, we used light microscopy and electron microscopy to examine Nestin-GFP transgenic mice and provide a detailed ultrastructural reconstruction analysis of Nestin-GFP-positive RGL cells of the dentate gyrus. We show that their primary processes follow a tortuous path from the subgranular zone through the granule cell layer and ensheathe local synapses and vasculature in the inner molecular layer. They share the ensheathing of synapses and vasculature with astrocytic processes and adhere to the adjacent processes of astrocytes. This extensive interaction of processes with their local environment could allow them to be uniquely receptive to signals from local neurons, glia, and vasculature, which may regulate their fate. |
| Databáze: | OpenAIRE |
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