A small element from the Mason-Pfizer monkey virus genome makes human immunodeficiency virus type 1 expression and replication Rev-independent
| Autor: | David Rekosh, Molly Bray, Susan Prasad, Kuan-Teh Jeang, Eric Hunter, John W. Dubay, Marie-Louise Hammarskjold |
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| Rok vydání: | 1994 |
| Předmět: |
Untranslated region
viruses DNA Mutational Analysis Gene Expression Gene Products gag Genome Viral Biology Regulatory Sequences Nucleic Acid Virus Replication Virus Retrovirus Sequence Homology Nucleic Acid MRNA transport RNA Messenger Nuclear export signal Cells Cultured Subgenomic mRNA Sequence Deletion Genetics Multidisciplinary RNA Gene Products env Biological Transport rev Gene Products Human Immunodeficiency Virus biology.organism_classification Virology Fusion Proteins gag-pol Gene Products rev Viral replication HIV-1 RNA Viral Mason-Pfizer monkey virus Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 91(4) |
| ISSN: | 0027-8424 |
| Popis: | Replication of human immunodeficiency virus type 1 (HIV-1) is dependent on the viral Rev protein. This protein acts in concert with the cis-acting rev-responsive element present in intron-containing RNAs to facilitate nuclear export of these RNAs. Here we show that a cis-acting 219-nucleotide sequence from an unrelated "simple" retrovirus, Mason-Pfizer monkey virus (MPMV), enables Rev-independent HIV-1 replication. This sequence is present in an untranslated region near the 3' end of the MPMV genome. The MPMV element is also able to efficiently substitute for Rev in expression of Gag/Pol and Env proteins from subgenomic constructs. We hypothesize that the MPMV element functions by interacting with a cellular factor that plays a role in mRNA transport analogous to that of the Rev protein. It might be possible to exploit this element in the development of an HIV vaccine. |
| Databáze: | OpenAIRE |
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