Fetal membranes act as a barrier for adenoviruses: gene transfer into exocoelomic cavity of rat fetuses does not affect cells in the fetus
| Autor: | Kari Vanamo, Seppo Ylä-Herttuala, Mervi S. Riekkinen, Seppo Heinonen, Annaleena Heikkilä, Anniina Laurema |
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| Rok vydání: | 2004 |
| Předmět: |
Indoles
Genetic enhancement Transgene Genetic Vectors Extraembryonic Membranes Gene Expression Biology Transfection Adenoviridae Andrology Fetus Pregnancy Fetal membrane Animals Transgenes Rats Wistar reproductive and urinary physiology Staining and Labeling Genetic transfer Gene Transfer Techniques Obstetrics and Gynecology Galactosides Embryonic stem cell Rats Chromogenic Compounds Lac Operon Giant cell In utero embryonic structures Immunology Female |
| Zdroj: | American Journal of Obstetrics and Gynecology. 190:264-267 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/j.ajog.2003.07.010 |
| Popis: | Objectives In utero gene therapy has a potential to correct genetic disorders before the first clinical symptoms appear. Our aim was to examine whether the exocoelomic cavity between amniotic and chorionic membranes offers a minimally invasive route for gene transfer to the fetus during early pregnancy. Study design We injected lacZ-adenovirus (4×109 pfu) during open surgery into the exocoelomic cavity of rat fetuses (n = 50) and analyzed the fetuses and rat dams for transgene expression with X-gal staining and polymerase chain reaction. Results Giant cells around Reichert's membrane, the outermost extraembryonic membrane in rodents, were transduced; but no transduction was observed in the cells of the fetuses or rat dams. Conclusion In rodents, the exocoelomic cavity does not offer a route for gene transfer into the fetus. It was concluded that fetal membranes act as a barrier that prevents adenoviral particles from passing between embryonic cavities. |
| Databáze: | OpenAIRE |
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