| Autor: |
Arvey, Aaron, Rowe, Michael, Legutki, Joseph Barten, An, Gang, Anantha Gollapudi, Lei, Anna, Colston, Bill, Putterman, Chaim, Smith, David, Stiles, Janelle, Tarasow, Theodore, Preveen Ramamoorthy |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.13059569.v1 |
| Popis: |
Additional file 8: Figure S8. Additional control experiments suggest that age-associated antibody-peptide binding is driven by direct IgG binding. (A) Original image associated with Fig. 4b. In addition to filter columns, we also demonstrated IgG separation with Melon Gel purification; however, the Melon Gel purification assay reagents disrupted IgG-peptide array binding in the recombined fractions (the positive control) even though it produced superior IgG purification. It was thus excluded from downstream analysis. (B) The age-associated probes are not bound by anti-IgG secondary antibody, which is used for detecting IgG bound to peptides. Generic stickiness or peptides with similarity to IgG-Fc (which can partially bind secondary-antibody directly; y-axis) have minimal correlation with on age-association (x-axis). Each data point is a probe. (C-D) Interfering substances found in varying abundances in serum have limited impact on age-associated peptide probes. Serum from 4 healthy donors with and without an interfering substance was assayed by peptide microarray. Triglycerides, rheumatoid factor (RF), conjugated bilirubin, human anti-mouse antibody (HAMA), hemoglobin, and unconjugated bilirubin at a single high concentration (Methods). (C) The log10 ratio of serum with and without interfering substance (y-axis) is compared to the log10 ratio of serum from older (> 60 years) and younger ( 0.25 and RF log10 ratio > 0.05; other cutoffs provided similar values). Pearson’s and Spearman correlation were not near significance, r = 0.06, rho = 0.03 (p 30) vs lower BMI ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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