Thermococcus barophilus sp. nov., a new barophilic and hyperthermophilic archaeon isolated under high hydrostatic pressure from a deep-sea hydrothermal vent
| Autor: | Dominique Marie, Viggo Marteinsson, Marc Vernet, Daniel Prieur, Jean-Louis Birrien, Paul Messner, Uwe B. Sleytr, Agata Gambacorta, Anna-Louise Reysenbach |
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| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Hydrostatic pressure Heterotroph DNA Ribosomal Microbiology RNA Ribosomal 16S Piezophile Hydrostatic Pressure Yeast extract Atlantic Ocean Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Genes rRNA Thermococcaceae Sequence Analysis DNA General Medicine biology.organism_classification Hyperthermophile Thermococcus Thermococcus barophilus DNA Archaeal Water Microbiology Nuclear chemistry |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 49:351-359 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/00207713-49-2-351 |
| Popis: | A novel barophilic, hyperthermophilic, anaerobic sulfur-metabolizing archaeon, strain MPT (T = type strain), was isolated from a hydrothermal vent site (Snakepit) on the Mid-Atlantic Ridge (depth, 3550 m). Enrichments and isolation were done under 40 MPa hydrostatic pressure at 95 degrees C. Strain MPT was barophilic at 75, 80, 85, 90, 95 and 98 degrees C, and was an obligate barophile between 95 and 100 degrees C (Tmax). For growth above 95 degrees C, a pressure of 15.0-17.5 MPa was required. The strain grew at 48-95 degrees C under atmospheric pressure. The optimal temperature for growth was 85 degrees C at both high (40 MPa) and low (0.3 MPa) pressures. The growth rate was twofold higher at 85 degrees C under in situ hydrostatic pressure compared to at low pressure. Strain MPT cells were motile, coccoid, 0.8-2.0 microns in diameter and covered by a hexagonal S-layer lattice. The optimum pH and NaCl concentration for growth at low pressure were 7.0 and 20-30 g l-1, respectively. The new isolate was an obligate heterotroph and utilized yeast extract, beef extract and peptone for growth. Growth was optimal in the presence of elemental sulfur. Rifampicin and chloramphenicol inhibited growth. The core lipids consisted of a major archaeol and a complex lipid pattern consisting of a major phospholipid. The DNA G + C content was 37.1 mol%. Sequencing of the 16S rRNA gene revealed that strain MPT belonged to the genus Thermococcus and it is proposed that this isolate should be designated as a new species, Thermococcus barophilus. |
| Databáze: | OpenAIRE |
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