EFFECT OF CYTOSTATIC AGENTS ON EXPRESSION LEVELS OF HUMAN BETA-DEFENSINS-1-4 IN A431 AND MCF-7 CELL LINES
| Autor: | O S Zubenko, I O Starenka, P V Pogribnyy, D O Semeniuk |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cisplatin Cancer Research Vincristine Chemistry Short communications 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Beta defensin Oncology Epidermoid carcinoma MCF-7 Mechanism of action Cell culture 030220 oncology & carcinogenesis medicine Cancer research medicine.symptom A431 cells medicine.drug |
| Zdroj: | Experimental Oncology. 40:79-81 |
| ISSN: | 2312-8852 1812-9269 |
| DOI: | 10.31768/2312-8852.2018.40(1):79-81 |
| Popis: | The aim of the study was to analyze an effect of cytostatic agents of different mechanism of action on expression levels of human beta-defensins-1-4 (hBD-1-4) in cultured human cancer cell lines. Materials and Methods: Expression levels of hBD-1-4 mRNA were assessed using qPCR in human epidermoid carcinoma A431 cells and human breast adenocarcinoma MCF7 cells treated with cisplatin, methotrexate, doxorubicin or vincristine at the IC20 concentrations. Results: The cytostatic agents with different mechanisms of action affected differently expression of hBDs, dependent on the cell line. Mostly, cytostatic agents suppressed significantly expression of hBDs. In contrast, vincristine caused significant up-regulation of hBD-1 (12 fold, p < 0.05) and hBD-4 (2 fold, p < 0.05) in MCF7, and doxorubicin significantly enhanced expression of hBD-3 (2 fold, p < 0.05) and hBD-4 (> 10 fold, p < 0.05) in A431 cells. Conclusion: The results of this pilot study show that expression levels of hBD-1-4 may be altered upon treatment with cytostatic agents depending on nature of cells. |
| Databáze: | OpenAIRE |
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