Circulating Tumor Cells Expressing Krüppel-Like Factor 8 and Vimentin as Predictors of Poor Prognosis in Pancreatic Cancer Patients
| Autor: | Peng Zhu, Hui-ying Liu, Sheng-Xian Yuan, Pan Zeya, Wei-Jun Wang, Fangming Gu, Jian Huang, Liu Fuchen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Adult
Male Poor prognosis recurrence pancreatic cancer Kruppel-Like Transcription Factors Vimentin Kaplan-Meier Estimate circulating tumor cells vimentin Circulating tumor cell Krüppel Risk Factors Pancreatic cancer Biomarkers Tumor medicine Humans Neoplasm Invasiveness Original Research Article RC254-282 biology Transition (genetics) business.industry Neoplasms. Tumors. Oncology. Including cancer and carcinogens Hematology General Medicine Middle Aged Krüppel-like factor 8 Neoplastic Cells Circulating Prognosis medicine.disease Phenotype Peripheral blood Pancreatic Neoplasms Oncology biology.protein Cancer research Female Neoplasm Recurrence Local business |
| Zdroj: | Cancer Control, Vol 28 (2021) Cancer Control : Journal of the Moffitt Cancer Center |
| ISSN: | 1073-2748 |
| DOI: | 10.1177/10732748211027163 |
| Popis: | Background: Circulating tumor cells (CTCs) with an epithelial-mesenchymal transition phenotype in peripheral blood may be a useful marker of carcinomas with poor prognosis. The aim of this study was to determine the prognostic significance of CTCs expressing Krüppel-like factor 8 (KLF8) and vimentin in pancreatic cancer (PC). Methods: CTCs were isolated by immunomagnetic separation from the peripheral blood of 40 PC patients before undergoing surgical resection. Immunocytochemistry was performed to identify KLF8+ and vimentin+ CTCs. The associations between CTCs and time to recurrence (TTR), clinicopathologic factors, and survival were assessed. Univariate and multivariate analyzes were performed to identify risk factors. Results: Patients with CTCs ( n = 30) had a higher relapse rate compared to those without ( n = 10) (70.0% vs 20.0%; P < 0.01). The proportion of KLF8+/vimentin+ CTCs to total CTCs was inversely related to TTR ( r = −0.646; P < 0.01); TTR was reduced in patients with > 50% of CTCs identified as KLF8+/vimentin+ ( P < 0.01). Independent risk factors for recurrence were perineural invasion and > 50% KLF8+/vimentin+ CTCs (both P < 0.05). Conclusion: Poor prognosis can be predicted in PC patients when > 50% of CTCs are positive for KLF8 and vimentin. |
| Databáze: | OpenAIRE |
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