Resveratrol Shows Vasoprotective Effect Reducing Oxidative Stress Without Affecting Metabolic Disturbances in Insulin-dependent Diabetes of Rabbits

Autor: Fatma Akar, Selen Soylemez, Can Tufan, M. Bilgehan Pektas, Kamile Ozturk, A. Tulga Ulus, Burcu Gokalp, H. Selcuk Surucu, Aylin Sepici
Přispěvatelé: Fen-Edebiyat Fakültesi, PEKTAS, MEHMET BILGEHAN -- 0000-0001-9790-8397, Akar, Fatma -- 0000-0002-5432-0304, SURUCU, HUSEYIN SELCUK -- 0000-0002-9244-4236
Rok vydání: 2011
Předmět:
Blood Glucose
Male
Endothelial and Vascular Function
Time Factors
medicine.medical_treatment
Resveratrol
medicine.disease_cause
Antioxidants
chemistry.chemical_compound
Alloxan
Stilbenes
Insulin
Testosterone
Pharmacology (medical)
Diabetic Rabbits
Lipid peroxide
biology
Superoxide
General Medicine
Catalase
Lipids
Rabbits
Cardiology and Cardiovascular Medicine
Lipid Peroxides
medicine.medical_specialty
Nitric Oxide Synthase Type III
Nitric Oxide
Diabetes Mellitus
Experimental
Superoxide dismutase
Internal medicine
Diabetes mellitus
medicine
Animals
Vascular Diseases
Pharmacology
Superoxide Dismutase
business.industry
Body Weight
Estrogens
medicine.disease
Acetylcholine
Vasoprotective
Oxidative Stress
Diabetes Mellitus
Type 1
Endocrinology
chemistry
biology.protein
business
NADP
Oxidative stress
Zdroj: Cardiovascular Drugs and Therapy. 25:119-131
ISSN: 1573-7241
0920-3206
Popis: WOS: 000290572200004
PubMed: 20676927
Resveratrol has been shown to have vasoprotective effects by upregulating oxidative defense mechanisms in a variety of pathophysiological conditions. However, the effect of resveratrol on diabetic oxidative stress and vascular and metabolic abnormalities is not completely understood. Therefore, this study was designed to evaluate whether long-term resveratrol supplementation has a protective effect on vascular function and integrity in association with metabolic parameters and oxidative stress in insulin-dependent diabetes. Diabetes was induced in rabbits with alloxan and maintained for 8 weeks. We used a resveratrol dose of 5 mg/L (10 weeks, starting 14 days before alloxan injection) and 50 mg/L (8 or 10 weeks, starting concomitantly or 14 days before alloxan injection) in the drinking water of rabbits. Relaxation to acetylcholine was impaired (control 75.6 +/- 3.59%, versus diabetic 42.23 +/- 2.53%) and contractions to phenylephrine increased (control 136.89 +/- 2.27%, versus diabetic 159.37 +/- 6.27%) in aortas from diabetic animals. These changes were associated with increased basal or NAD(P)H-induced superoxide production, as well as lipid peroxide and superoxide dismutase (SOD) levels in the aortic samples. The maximal relaxation to acetylcholine improved by 75.74 +/- 9.04% in diabetic rabbits treated with resveratrol. The increased contractions to phenylephrine were not restored to control values after resveratrol treatments, but sensitivity to the contractions tended to decrease. Resveratrol increased nitrite/nitrate levels and suppressed basal or NAD(P)H-induced superoxide production and lipid peroxide levels in the aortas. Importantly, resveratrol increased serum insulin levels without affecting blood glucose and the lipid profile in diabetic rabbits. Using electron microscopic examinations, resveratrol was found to markedly protect the endothelial integrity from diabetes. Overall, there was no noticeable difference between resveratrol treatment groups on the recovery from diabetes. Our results indicate that resveratrol alleviates type 1 diabetes-induced vasculopathy by decreasing vascular oxidative stress and thereby increasing the bioavailability of nitric oxide without changing metabolic abnormalities.
TUBITAK [105 S431]
This study was supported by a grant from TUBITAK 2008, Project No: 105 S431.
Databáze: OpenAIRE
Externí odkaz: