Culture of human corneal endothelial cells isolated from corneas with Fuchs endothelial corneal dystrophy
| Autor: | Karine Zaniolo, Stéphanie Proulx, Marie-Claude Perron, A. Deschambeault, Cristina Bostan, Olivier Drouin, Isabelle Brunette |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Aging Pathology medicine.medical_specialty Endothelium Cell Culture Techniques Cell Separation Biology Cell morphology 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine medicine Humans Fluorescent Antibody Technique Indirect Cell Shape Descemet Membrane Aged Cell Proliferation 030304 developmental biology Aged 80 and over 0303 health sciences Clusterin Cell growth Endothelium Corneal Fuchs' Endothelial Dystrophy Anatomy Middle Aged eye diseases Sensory Systems In vitro Descemet's membrane Ophthalmology medicine.anatomical_structure Cell culture 030221 ophthalmology & optometry biology.protein Keratins Female sense organs Biomarkers Fuchs Endothelial Corneal Dystrophy |
| Zdroj: | Experimental Eye Research. 94:22-31 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2011.10.018 |
| Popis: | The purpose of this study was to assess the feasibility of initiating primary cultures of corneal endothelial cells from patients suffering from Fuchs endothelial corneal dystrophy (FECD; MIM# 1036800). We also evaluated which conditions yielded the best results for culture. Twenty-nine patients undergoing Descemet stripping automated endothelial keratoplasty consented to the use of their excised Descemet's membrane for this study. Out of the 29 specimens, 18 successfully initiated a culture. Cell morphology varied between endothelial (rounded, slightly elongated cells, n = 12) and fibroblastic-like (thin and very elongated cells, n = 6). These differences in cell morphology were also observed with the normal human corneal endothelial cell cultures. The cultures that initially presented an endothelial morphology maintained their shape in subcultures. Clusterin expression was similar in FECD and normal endothelial cells. Transmission electron microscopy of FECD Descemet's membranes showed a high degree of various abnormalities generally found in this disease, such as a thickened Descemet's membrane, presence of a posterior banded layer, presence of a fibrillar layer and striated bodies of various sizes and periodicities. Patient's age was predictive of culture success, all younger FECD donors generating cultures of endothelial morphology. The absence of a fibrillar layer was also a factor associated with greater success. Culture success was not dependent on specimen size, specimen pigmentation, or patient's preoperative central corneal thickness. In conclusion, this paper shows for the first time that central Descemet's membranes of patients suffering from FECD possess proliferative endothelial cells that can be isolated and cultured without viral transduction, opening the way for new in vitro studies of this disease. |
| Databáze: | OpenAIRE |
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