In vivo and in vitro studies of cartilage differentiation in altered gravities
| Autor: | P.J Duke, Dina Montufar-Solis, D D'Aunno |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Atmospheric Science
Time Factors Mesenchyme Epiphyseal plate Morphogenesis Aerospace Engineering Centrifugation Hypergravity Matrix (biology) Rats Sprague-Dawley Immobilization In vivo medicine Animals Growth Plate Weightlessness Simulation Gravity Altered Tibia Weightlessness Chemistry Cartilage Cell Differentiation Astronomy and Astrophysics Space Flight Chondrogenesis In vitro Extracellular Matrix Hindlimb Rats Cell biology Geophysics medicine.anatomical_structure Space and Planetary Science General Earth and Planetary Sciences Female Cell Division |
| Zdroj: | Advances in Space Research. 17:193-199 |
| ISSN: | 0273-1177 |
| DOI: | 10.1016/0273-1177(95)00635-r |
| Popis: | The in vivo model our laboratory uses for studies of cartilage differentiation in space is the rat growth plate. Differences between missions, and in rat age and recovery times, provided differing results from each mission. However, in all missions, proliferation and differentiation of chondrocytes in the epiphyseal plate of spaceflown rats was altered as was matrix organization. In vitro systems, necessary complements to in vivo work, provide some advantages over the in vivo situation. In vitro , centrifugation of embryonic limb buds suppressed morphogenesis due to precocious differentiation, and changes in the developmental pattern suggest the involvement of Hox genes. In space, embryonic mouse limb mesenchyme cells differentiating in vitro on IML-1 had smoother membranes and lacked matrix seen in controls. Unusual formations, possibly highly ruffled membranes, were found in flight cultures. These results, coupled with in vivo centrifugation studies, show that in vivo or in vitro , the response of chondrocytes to gravitational changes follows Hert's curve as modified by Simon, i.e. decreased loading decreases differentiation, and increased loading speeds it up, but only to a point. After that, additional increases again slow down chondrogenesis. |
| Databáze: | OpenAIRE |
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