Recent Origin of the Methacrylate Redox System in Geobacter sulfurreducens AM-1 through Horizontal Gene Transfer
| Autor: | Galina V. Mikoulinskaia, Marina V. Zakharova, Alexander Galushko, O. V. Arkhipova, Fyodor A. Kondrashov, Margarita Meer, Vasilii K. Akimenko |
|---|---|
| Přispěvatelé: | Томский государственный университет Институт биологии, экологии, почвоведения, сельского и лесного хозяйства (Биологический институт) Кафедра физиологии растений и биотехнологии |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
метакрилатные системы
Evolució molecular Gene Transfer Horizontal Operon Sequence analysis Science Molecular Sequence Data Genome гены Bacterial Proteins Molecular evolution Gene Order Geobacter sulfurreducens Gene Phylogeny Genetics Multidisciplinary biology food and beverages biology.organism_classification горизонтальный перенос генов Biological Evolution Horizontal gene transfer Medicine Methacrylates Geobacter Oxidation-Reduction Genome Bacterial Research Article |
| Zdroj: | PLoS ONE PLoS ONE. 2015. Vol. 10, № 5. P. e0125888 (1-15) Recercat. Dipósit de la Recerca de Catalunya instname PLoS ONE, Vol 10, Iss 5, p e0125888 (2015) |
| ISSN: | 1932-6203 |
| Popis: | The origin and evolution of novel biochemical functions remains one of the key questions in molecular evolution. We study recently emerged methacrylate reductase function that is thought to have emerged in the last century and reported in Geobacter sulfurreducens strain AM-1. We report the sequence and study the evolution of the operon coding for the flavin-containing methacrylate reductase (Mrd) and tetraheme cytochrome с (Mcc) in the genome of G. sulfurreducens AM-1. Different types of signal peptides in functionally interlinked proteins Mrd and Mcc suggest a possible complex mechanism of biogenesis for chromoproteids of the methacrylate redox system. The homologs of the Mrd and Mcc sequence found in δ-Proteobacteria and Deferribacteres are also organized into an operon and their phylogenetic distribution suggested that these two genes tend to be horizontally transferred together. Specifically, the mrd and mcc genes from G. sulfurreducens AM-1 are not monophyletic with any of the homologs found in other Geobacter genomes. The acquisition of methacrylate reductase function by G. sulfurreducens AM-1 appears linked to a horizontal gene transfer event. However, the new function of the products of mrd and mcc may have evolved either prior or subsequent to their acquisition by G. sulfurreducens AM-1. The work has been supported by a grant of the HHMI International Early Career Scientist Program (55007424), the Spanish Ministry of Economy and Competitiveness (EUI-EURYIP-2011-4320) as part of the EMBO YIP program, two grants from the Spanish Ministry of Economy and Competitiveness, "Centro de Excelencia Severo Ochoa 2013–2017 (Sev-2012-0208)" and (BFU2012-31329), the European Union and the European Research Council under grant agreement/n335980_EinME. |
| Databáze: | OpenAIRE |
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