Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae
| Autor: | J. D. Beggs, Penny R. Hirsch |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Octopine
Saccharomyces cerevisiae Agrobacterium Agrobacterium tumefaciens Biology Molecular cloning biology.organism_classification Molecular biology Yeast Gall Saccharomyces Ti plasmid chemistry.chemical_compound Plasmid Tumour cell chemistry Shuttle vector Genetics Saccharomyces Cerevisiae Molecular Biology |
| Zdroj: | Molecular and General Genetics MGG. 195:209-214 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00332748 |
| Popis: | A section of the octopine Ti plasmid pTiACH5 including the region always integrated in crown gall tumour cells (the TL-DNA) was cloned into the yeast — E. coli shuttle vector pJDB207. This plasmid, pOTY8, was transformed into S. cerevisiae where transcription of the T-DNA was demonstrated, but there was no evidence for the production of octopine synthetase. The TL-DNA was cloned into a derivative of pJDB207 unable to replicate in yeast, but the TL-DNA did not appear to contain any autonomously replicating sequences (ars) functional in yeast. |
| Databáze: | OpenAIRE |
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