Spermatogenesis in the Cynomolgus Monkey (Macaca fascicularis): A Practical Guide for Routine Morphological Staging
| Autor: | Eric Van Esch, Eveline P. C. T. de Rijk, Henriette C. Dreef |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Stage classification Pathology medicine.medical_specialty 040301 veterinary sciences Guidelines as Topic Biology Testicle Toxicology 030226 pharmacology & pharmacy Male Reproductive Tract Pathology and Forensic Medicine 0403 veterinary science Fixatives 03 medical and health sciences 0302 clinical medicine Testis Paraffin section medicine Animals Paraffin embedding Hematoxylin Spermatogenesis Molecular Biology Paraffin Embedding Sertoli Cells Staining and Labeling Histocytochemistry 04 agricultural and veterinary sciences Cell Biology Periodic Acid-Schiff Reaction Seminiferous Tubules Spermatozoa Spermatogonia Macaca fascicularis medicine.anatomical_structure Testis tissue Eosine Yellowish-(YS) |
| Zdroj: | Toxicologic Pathology. 35:395-404 |
| ISSN: | 1533-1601 0192-6233 |
| DOI: | 10.1080/01926230701230346 |
| Popis: | The cynomolgus monkey ( Macaca fascicularis) is widely used in regulatory toxicity studies. Especially in studies on male contraception, the male reproductive tract can be an important target system. The aim of the present paper is to describe a practical approach for morphological staging of spermatogenesis in routinely prepared paraffin sections. Results obtained using this approach could help to determine possible drug-related effects on spermatogenesis. As a guide to the investigators, photomicrographs of Bouin-fixed, paraffin-embedded and H&E or PAS stained sections from testis tissue are presented to illustrate the twelve successive morphological stages (cell associations) of normal spermatogenesis. Sexually immature or peripubertal monkeys sometimes are included in toxicity studies. Since the morphological features of the testes of such monkeys can be mistaken for treatment-related abnormalities, the morphologic characteristics of these testes are described and discussed briefly. |
| Databáze: | OpenAIRE |
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