Mouse Offspring after Microinjection of Heated Spermatozoa
| Autor: | N Lièvre, Morgane Bomsel, Frédérique Monier-Gavelle, Jean-Philippe Wolf, J Cozzi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
endocrine system Hot Temperature Microinjections Offspring medicine.medical_treatment Biology Intracytoplasmic sperm injection Andrology Embryonic and Fetal Development Mice Human fertilization Pregnancy medicine Animals Sperm Injections Intracytoplasmic Blastocyst Microinjection reproductive and urinary physiology Genetics urogenital system Embryogenesis Pregnancy Outcome Embryo Cell Biology General Medicine Embryo Transfer Spermatozoa Sperm medicine.anatomical_structure Reproductive Medicine Female |
| Zdroj: | Biology of Reproduction. 65:1518-1521 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod65.5.1518 |
| Popis: | The thermostability of the mammalian sperm genome was previously reported, but no live offspring after conception with heated spermatozoa had yet been obtained. In the present study, mouse spermatozoa were heated at 56 degrees C for 30 min and microinjected into mouse oocytes. Fertilization did not occur unless activation was induced by incubation in a calcium-free medium containing strontium. Under these conditions fertilization and cleavage rates were comparable to those obtained after microinjection of control spermatozoa, but the developmental rate to the blastocyst stage was lower. When transferred to foster mothers, embryos derived from heated sperm developed into phenotypically normal offspring, which grew and reproduced normally. In the mouse, heated spermatozoa can therefore support full embryonic development after microinjection into oocytes. |
| Databáze: | OpenAIRE |
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