Plasma Exosomes Contribute to Microvascular Damage in Diabetic Retinopathy by Activating the Classical Complement Pathway
| Autor: | Kiera Fisher, Chao Huang, Julia V. Busik, Sandra S Hammer, Svetlana Navitskaya, Gary J. Blanchard |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Complications Endocrinology Diabetes and Metabolism Vascular permeability Exosomes Retina Diabetes Mellitus Experimental Capillary Permeability 03 medical and health sciences Classical complement pathway chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission Diabetes mellitus Centrifugation Density Gradient Internal Medicine medicine Extracellular Animals Complement Activation Mice Knockout Diabetic Retinopathy business.industry Retinal Vessels Retinal Complement System Proteins Diabetic retinopathy medicine.disease Microvesicles 3. Good health Complement system Mice Inbred C57BL 030104 developmental biology Microscopy Fluorescence chemistry Immunoglobulin G 030220 oncology & carcinogenesis Microvessels Disease Progression Cancer research business Ultracentrifugation Biomarkers |
| Zdroj: | Diabetes |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/db17-1587 |
| Popis: | Diabetic retinopathy (DR) is a microvascular complication of diabetes and is the leading cause of vision loss in working-age adults. Recent studies have implicated the complement system as a player in the development of vascular damage and progression of DR. However, the role and activation of the complement system in DR are not well understood. Exosomes, small vesicles that are secreted into the extracellular environment, have a cargo of complement proteins in plasma, suggesting that they can participate in causing the vascular damage associated with DR. We demonstrate that IgG-laden exosomes in plasma activate the classical complement pathway and that the quantity of these exosomes is increased in diabetes. Moreover, we show that a lack of IgG in exosomes in diabetic mice results in a reduction in retinal vascular damage. The results of this study demonstrate that complement activation by IgG-laden plasma exosomes could contribute to the development of DR. |
| Databáze: | OpenAIRE |
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