Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein
| Autor: | Brian A. Morgan, Zoulfia Darieva, Steven G. Sedgwick, Richard Bulmer, Marco Geymonat, Kathryn S. Doris, Aline Pic-Taylor, Andrew D. Sharrocks |
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| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Multidisciplinary Models Genetic Transcription Genetic Kinase Cell Cycle Polo kinase Transcription factor complex Cell Cycle Proteins Promoter Saccharomyces cerevisiae Cyclin B Protein Serine-Threonine Kinases Cell cycle Biology Article Cell biology Gene Expression Regulation Fungal Coactivator Serine Phosphorylation Promoter Regions Genetic Protein Kinases Mitosis Transcription Factors |
| Zdroj: | Nature. 444:494-498 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/nature05339 |
| Popis: | Polo kinases play crucial conserved roles in controlling the eukaryotic cell cycle through orchestrating several events during mitosis1,2. An essential element of cell cycle control is exerted by altering the expression of key regulators3. Here, we demonstrate an important role for the polo kinase, Cdc5p, in controlling cell cycle-dependent gene expression which is critical for the execution of mitosis in the model eukaryote Saccharomyces cerevisiae. In particular, we find that Cdc5p is temporally recruited to promoters of the cell cycle-regulated CLB2 gene cluster, where it targets the Mcm1p-Fkh2p-Ndd1p transcription factor complex, through direct phosphorylation of the coactivator protein Ndd1p. This phosphorylation event is required for the normal temporal expression of cell cycle-regulated genes such as CLB2 and SWI5 in G2/M phases. Furthermore, severe defects in cell division occur in the absence of Cdc5p-mediated phosphorylation of Ndd1p. Thus, polo kinase is required for the production of key mitotic regulators, in addition to previously defined roles in controlling other mitotic events. |
| Databáze: | OpenAIRE |
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