Degradation of Sargassum crassifolium Fucoidan by Ascorbic Acid and Hydrogen Peroxide, and Compositional, Structural, and In Vitro Anti-Lung Cancer Analyses of the Degradation Products
| Autor: | Chun-Yung Huang, Chia-Hung Kuo, Tien-Chiu Wu, Shu-Ling Hsieh, Ren-Han Huang, Yung-Hsiang Tsai, Yong-Han Hong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pharmaceutical Science
hydrogen peroxide brown algae 01 natural sciences 03 medical and health sciences chemistry.chemical_compound fucoidan anti-lung cancer Drug Discovery Hydrogen peroxide Cytotoxicity Pharmacology Toxicology and Pharmaceutics (miscellaneous) lcsh:QH301-705.5 PI3K/AKT/mTOR pathway 030304 developmental biology 0303 health sciences biology 010405 organic chemistry Fucoidan Cytochrome c apoptosis Ascorbic acid In vitro 0104 chemical sciences human lung carcinoma A-549 cells chemistry Biochemistry lcsh:Biology (General) Apoptosis biology.protein Sargassum crassifolium ascorbic acid |
| Zdroj: | Marine Drugs Volume 18 Issue 6 Marine Drugs, Vol 18, Iss 334, p 334 (2020) |
| ISSN: | 1660-3397 |
| DOI: | 10.3390/md18060334 |
| Popis: | Fucoidans possess multiple biological functions including anti-cancer activity. Moreover, low-molecular-weight fucoidans are reported to possess more bioactivities than native fucoidans. In the present study, a native fucoidan (SC) was extracted from Sargassum crassifolium pretreated by single-screw extrusion, and three degraded fucoidans, namely, SCA (degradation of SC by ascorbic acid), SCH (degradation of SC by hydrogen peroxide), and SCAH (degradation of SC by ascorbic acid + hydrogen peroxide), were produced. The extrusion pretreatment can increase the extraction yield of fucoidan by approximately 4.2-fold as compared to the non-extruded sample. Among SC, SCA, SCH, and SCAH, the chemical compositions varied but structural features were similar. SC, SCA, SCH, and SCAH showed apoptotic effects on human lung carcinoma A-549 cells, as illustrated by loss of mitochondrial membrane potential (MMP), decreased B-cell leukemia-2 (Bcl-2) expression, increased cytochrome c release, increased active caspase-9 and -3, and increased late apoptosis of A-549 cells. In general, SCA was found to exhibit high cytotoxicity to A-549 cells and a strong ability to suppress Bcl-2 expression. SCA also showed high efficacy to induce cytochrome c release, activate caspase-9 and -3, and promote late apoptosis of A-549 cells. Therefore, our data suggest that SCA could have an adjuvant therapeutic potential in the treatment of lung cancer. Additionally, we explored that the Akt/mammalian target of rapamycin (mTOR) signaling pathway is involved in SC-, SCA-, SCH-, and SCAH-induced apoptosis of A-549 cells. |
| Databáze: | OpenAIRE |
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