Blockade of glutamate release by botulinum neurotoxin type A in humans:A dermal microdialysis study
| Autor: | Sten Rasmussen, Ali Karshenas, Flemming W. Bach, Parisa Gazerani, Lars Arendt-Nielsen, Larissa Bittencourt da Silva |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Skin Temperature/drug effects
Adult Male Microdialysis Analgesic Neuropeptide Glutamic Acid Pain Vasodilation Pharmacology Administration Cutaneous chemistry.chemical_compound Young Adult Pain/drug therapy Medicine Humans Botulinum Toxins Type A lcsh:R5-920 Microdialysis/methods Botulinum Neurotoxin Type A business.industry Vasodilation/drug effects Botulinum Toxins Type A/administration & dosage Glutamate receptor Blockade Anesthesiology and Pain Medicine Neurology chemistry Glutamic Acid/metabolism Capsaicin Anesthesia Female Original Article lcsh:Medicine (General) business Skin Temperature |
| Zdroj: | da Silva, L B, Karshenas, A, Bach, F W, Rasmussen, S, Arendt-Nielsen, L & Gazerani, P 2014, ' Blockade of glutamate release by botulinum neurotoxin type A in humans : A dermal microdialysis study ', Pain Research & Management, vol. 19, no. 3, pp. 126-132 . https://doi.org/10.1155/2014/410415 Scopus-Elsevier Europe PubMed Central Pain Research and Management, Vol 19, Iss 3, Pp 126-132 (2014) Aarhus University Bittencourt da Silva, L, Karshenas, A, Bach, F W, Rasmussen, S, Arendt-Nielsen, L & Gazerani, P 2014, ' Blockade of glutamate release by botulinum neurotoxin type A in humans: a dermal microdialysis study ', Pain Research & Management, vol. 19, no. 3, pp. 126-32 . |
| DOI: | 10.1155/2014/410415 |
| Popis: | BACKGROUND: The analgesic action of botulinum neurotoxin type A (BoNTA) has been linked to the blockade of peripheral release of neuropeptides and neurotransmitters in animal models; however, there is no direct evidence of this in humans.OBJECTIVES: To investigate the effect of BoNTA on glutamate release in humans, using an experimental model of pain and sensitization provoked by capsaicin plus mild heat.METHODS: Twelve healthy volunteers (six men, six women) were pretreated with BoNTA (10 U) on the volar forearm and with a saline control on the contralateral side. Dermal microdialysis was applied one week later to collect interstitial samples before and after the application of a capsaicin patch (8%) plus mild heat (40°C/60 min) to provoke glutamate release, pain and vasodilation. Samples were collected every hour for 3 h using linear microdialysis probes (10 mm, 100 kD). Dialysate was analyzed for glutamate concentration. Pain intensity and skin vasomotor reactions (temperature and blood flow changes) were also recorded.RESULTS: BoNTA significantly reduced glutamate release compared with saline (PCONCLUSIONS: The present study provided the first direct evidence supporting the inhibitory effect of BoNTA on glutamate release in human skin, which is potentially responsible for some of the analgesic action of BoNTA. |
| Databáze: | OpenAIRE |
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