Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand
| Autor: | Chaiyaporn Chaisatit, Sorayut Chattrakarn, Michele D. Spring, Jariyanart Gaywee, Krisada Jongsakul, Wuttikon Rodkvamtook, Montri Arsanok, Douglas Walsh, Paphavee Lertsethtakarn, Piyaporn Saingam, Mariusz Wojnarski, Chatchadaporn Thamnurak, Chantida Praditpol, Worachet Kuntawunginn, Watcharintorn Fagnark, Winita Ta-aksorn, Mark M. Fukuda, Charlotte A. Lanteri, Suwanna Chaorattanakawee, Panita Gosi, Norman C. Waters, Siratchana Sundrakes, David L. Saunders, Brian Vesely, Pattaraporn Vanachayangkul, Darunee Utainnam, Nonlawat Boonyalai, Kirakarn Kirativanich, Narongrid Sirisopana, Nichapat Uthaimongkol, Philip L. Smith |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Endemic Diseases Drug Resistance Protozoan Proteins Parasitemia medicine.disease_cause law.invention 0302 clinical medicine Genotype-phenotype distinction law Malaria Falciparum Genetics Mutation Multidisciplinary Middle Aged Thailand Artemisinins Transmission (mechanics) Quinolines Medicine Drug Therapy Combination Female Adult Adolescent DNA Copy Number Variations Genotype Science Plasmodium falciparum 030106 microbiology 030231 tropical medicine Biology Article Antimalarials Young Adult 03 medical and health sciences Piperaquine parasitic diseases medicine Humans Parasite genetics Genetic Association Studies Aged Point mutation Haplotype DNA Protozoan medicine.disease biology.organism_classification Malaria Haplotypes |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions. |
| Databáze: | OpenAIRE |
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