MMP-12 and S100s in saliva reflect different aspects of periodontal inflammation
| Autor: | Sigvard Åkerman, Mirjam Majster, Anders Gustafsson, Stephanie Zwicker, Elisabeth A. Boström, Björn Klinge, Sofia Björnfot Holmström, Mattias Svensson, Ronaldo Lira-Junior |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Saliva Periodontal tissue Immunology Bleeding on probing Alveolar Bone Loss Physiology Enzyme-Linked Immunosorbent Assay Matrix metalloproteinase Biochemistry S100A8 03 medical and health sciences 0302 clinical medicine Matrix Metalloproteinase 12 Immunology and Allergy Medicine Humans Molecular Biology Periodontal Diseases Periodontitis Inflammation business.industry S100 Proteins Smoking Gingival Pocket Hematology Middle Aged medicine.disease 030104 developmental biology 030220 oncology & carcinogenesis Female Calprotectin medicine.symptom business Leukocyte L1 Antigen Complex Biomarkers |
| Zdroj: | Cytokine. 113 |
| ISSN: | 1096-0023 |
| Popis: | Matrix metalloproteinase (MMP)-12, S100A8/A9, and S100A12 are involved in innate immune responses. We addressed whether different aspects of oral health and non-disease-related covariates influence their levels in saliva. 436 participants were clinically examined, completed a health questionnaire, and provided stimulated saliva. Salivary levels of MMP-12, S100A8/A9, and S100A12 were determined by enzyme-linked immunosorbent assays. Lower MMP-12 levels were observed in individuals 40-64 years old (yo) compared to40 yo, and higher S100A8/A9 levels were found in individuals64 yo compared to 40-64 yo. Smokers exhibited lower MMP-12 and S100A12 levels compared to non-smokers. All three proteins were elevated in individuals with bleeding on probing (BOP) 20% compared to those with BOP ≤ 20%, and the S100A8/A9 levels were higher in individuals having ≥ 10% gingival pocket depths (PPD) ≥ 4 mm compared to the ones with shallow pockets4 mm. The extent of alveolar bone loss or presence of manifest caries did not alter any of the markers. MMP-12, S100A8/A9, and S100A12 levels were higher in participants with high periodontal inflammatory burden. All three proteins correlated positively to BOP, PPD, and to several inflammatory mediators. The explanatory variables for MMP-12 in saliva were age, smoking, presence of any tumor, and percentage of PPD ≥ 4 mm. The determinant of salivary S100A8/A9 was percentage of BOP, while S100A12 levels were associated with percentage of BOP and presence of any tumor. Taken together, MMP-12 and the S100/calgranulin levels in saliva reflect different aspects of periodontal inflammation. Smoking and age should be taken into account in further investigation of these proteins as biomarker candidates of periodontal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect