DNA insecticide developed from the Lymantria dispar 5.8S ribosomal RNA gene provides a novel biotechnology for plant protection
Autor: | Alisa M. Krasnodubets, Iryna I Fomochkina, Kubyshkin Av, Ilya A. Novikov, Yuri I. Gninenko, Refat Z. Useinov, Valeriy V. Dyadichev, Tatiana Pavlovna Makalish, Evgenia Y. Bessalova, Volodymyr V. Oberemok, Zenure Z. Temirova, Anna I. Repetskaya, Kateryna V. Laikova, Nikita V Gal'chinsky, Maksym N. Shumskykh |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Insecticides Dispar media_common.quotation_subject 5.8S ribosomal RNA lcsh:Medicine Insect Moths Article Model invertebrates Oligodeoxyribonucleotides Antisense 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lymantria dispar Animals lcsh:Science Gene media_common Multidisciplinary biology Oligonucleotide business.industry fungi lcsh:R RNA Genes rRNA DNA biology.organism_classification RNA Ribosomal 5.8S Biotechnology 030104 developmental biology chemistry Larva lcsh:Q business PCR-based techniques 030217 neurology & neurosurgery |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Having observed how botanicals and other natural compounds are used by nature to control pests in the environment, we began investigating natural polymers, DNA and RNA, as promising tools for insect pest management. Over the last decade, unmodified short antisense DNA oligonucleotides have shown a clear potential for use as insecticides. Our research has concentrated mainly on Lymantria dispar larvae using an antisense oligoRING sequence from its inhibitor-of-apoptosis gene. In this article, we propose a novel biotechnology to protect plants from insect pests using DNA insecticide with improved insecticidal activity based on a new antisense oligoRIBO-11 sequence from the 5.8S ribosomal RNA gene. This investigational oligoRIBO-11 insecticide causes higher mortality among both L. dispar larvae grown in the lab and those collected from the forest; in addition, it is more affordable and faster acting, which makes it a prospective candidate for use in the development of a ready-to-use preparation. |
Databáze: | OpenAIRE |
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