Distinct regulation of gene expression in human endothelial cells by TGF-beta and its receptors
| Autor: | Nanping Wang, Jing-Dong Han, Xiaoping Wu, Jing Ma, Ye-Guang Chen |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Angiogenesis
Receptor Transforming Growth Factor-beta Type I Neovascularization Physiologic Biology Protein Serine-Threonine Kinases Biochemistry Adenoviridae Genes Reporter Transforming Growth Factor beta Gene expression Humans Receptor Luciferases Regulation of gene expression Microarray analysis techniques Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cell Biology Transforming growth factor beta Microarray Analysis Cell biology Gene expression profiling Gene Expression Regulation biology.protein Endothelium Vascular Signal transduction Cardiology and Cardiovascular Medicine Activin Receptors Type I Receptors Transforming Growth Factor beta Signal Transduction |
| Zdroj: | Microvascular research. 71(1) |
| ISSN: | 0026-2862 |
| Popis: | Transforming growth factor beta (TGF-beta) and its signaling mediators play essential roles in angiogenesis-formation of new blood vessels, as evidenced by targeted gene disruption in mice and their mutations in human vascular dysplasia. However, little is known about the molecular basis of TGF-beta function in vascular formation. To study the function of TGF-beta signaling in angiogenesis and to elucidate the signaling specificity of TGF-beta receptors at the gene transcriptional level, we analyzed the expression profile of the genes regulated by TGF-beta and its type I receptors ALK1 and ALK5 in human microvessel endothelial cells (ECs). Global change of gene expression profiles was examined by microarray and RT-PCR analyses in the ECs treated with TGF-beta1 or by adenoviral expression of the active ALK1 or ALK5. We found that the profiles of the genes regulated by TGF-beta, ALK1 and ALK5 are distinct from each other, although some of genes are modulated by all of them. TGF-beta regulated far more genes than ALK1 and ALK5 did. ALK1 enhanced the formation of tube-like structures of ECs, while ALK5 stimulates EC aggregation. Our results suggest that ALK1 appears to have important functions in regulating proliferation of ECs, whereas ALK5 tends to modulate cell-cell interaction and cell adhesion and extracellular matrix remodeling. |
| Databáze: | OpenAIRE |
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