Foam separation of Rhodamine-G and Evans Blue using a simple separatory bottle system
| Autor: | Yoichiro Ito, Dhweeja Dasarathy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
business.product_category
Nitrogen 0211 other engineering and technologies Analytical chemistry 02 engineering and technology Foam separation Biochemistry Article Chemistry Techniques Analytical Analytical Chemistry Rhodamine chemistry.chemical_compound Surface-Active Agents Pulmonary surfactant Bottle Sodium dodecyl sulfate 021101 geological & geomatics engineering Evans Blue Chromatography Rhodamines Organic Chemistry Sodium Dodecyl Sulfate General Medicine 021001 nanoscience & nanotechnology Solvent chemistry Volume (thermodynamics) Solvents 0210 nano-technology business |
| Popis: | A simple separatory glass bottle was used to improve separation effectiveness and cost efficiency while simultaneously creating a simpler system for separating biological compounds. Additionally, it was important to develop a scalable separation method so this would be applicable to both analytical and preparative separations. Compared to conventional foam separation methods, this method easily forms stable dry foam which ensures high purity of yielded fractions. A negatively charged surfactant, sodium dodecyl sulfate (SDS), was used as the ligand to carry a positively charged Rhodamine-G, leaving a negatively charged Evans Blue in the bottle. The performance of the separatory bottle was tested for separating Rhodamine-G from Evans Blue with sample sizes ranged from 1 to 12 mg in preparative separations and 1–20 μg in analytical separations under optimum conditions. These conditions including N 2 gas pressure, spinning speed of contents with a magnetic stirrer, concentration of the ligand, volume of the solvent, and concentration of the sample, were all modified and optimized. Based on the calculations at their peak absorbances, Rhodamine-G and Evans Blue were efficiently separated in times ranging from 1 h to 3 h, depending on sample volume. Optimal conditions were found to be 60 psi N 2 pressure and 2 mM SDS for the affinity ligand. This novel separation method will allow for rapid separation of biological compounds while simultaneously being scalable and cost effective. |
| Databáze: | OpenAIRE |
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