Characterizing forensically important insect and microbial community colonization patterns in buried remains
| Autor: | Cristina Purcarea, Georgiana Necula-Petrareanu, Emily N. Junkins, Lavinia Iancu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Insecta media_common.quotation_subject Science Biodiversity Colony Count Microbial Insect Biology Decomposer Article 03 medical and health sciences 0302 clinical medicine Abundance (ecology) RNA Ribosomal 16S Intestine Small Animals Colonization 030216 legal & forensic medicine media_common Soil Gravity Principal Component Analysis Multidisciplinary Ecology Diptera Microbiota Chemical process of decomposition Forensic Sciences Temperature Clostridium Paraputrificum Rats Coleoptera 030104 developmental biology Buried Remains Microbial population biology Advanced Decay Stages Postmortem Changes Principal component analysis Medicine PMI Estimation |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-16 (2018) |
| ISSN: | 2045-2322 |
| Popis: | During violent criminal actions in which the perpetrator disposes of the victim’s remains by burial, the analysis of insects and bacterial colonization patterns could be necessary for postmortem interval (PMI) estimation. Our research aimed to assess the decomposition process of buried rat carcasses from shallow graves (40 cm), the diversity and dynamics of insects and bacteria throughout the decomposition stages, and the environmental parameters’ influence on these variations. The results provide further insight on decomposition in soil and contribute to a broader understanding of the factors involved in decomposition by qualitatively and quantitatively analysing the decomposer community (bacteria and insects). Additionally, two bacterial taxa, Enterococcus faecalis and Clostridium paraputrificum that were investigated for the first time as PMI indicators using quantitative polymerase chain reaction (qPCR) showed differential abundance over time, promising data for PMI estimation. The current study on the decomposition of buried rat carcasses in a natural environment will strengthen the current knowledge on decomposed remains from shallow graves and represents an effort to quantify insect and bacterial taxa as PMI estimators. |
| Databáze: | OpenAIRE |
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