Production of Rubella Hemagglutinating Antigen in Suspension Culture
| Autor: | Gary L. Gitnick, A. Auletta, Anita C. Ley, Monroe M. Vincent, John L. Sever, N Tzan |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Virus Cultivation
General Immunology and Microbiology Rubella virus Articles Hemagglutination Tests General Medicine Biology medicine.disease medicine.disease_cause Suspension culture Virology Rubella General Biochemistry Genetics and Molecular Biology Microbiology Titer Antigen Culture Techniques medicine Baby hamster kidney cell Animals Antigens General Pharmacology Toxicology and Pharmaceutics Fetal bovine serum |
| Zdroj: | Applied Microbiology. 15:1244-1247 |
| ISSN: | 0003-6919 |
| DOI: | 10.1128/am.15.5.1244-1247.1967 |
| Popis: | Rubella virus specific hemagglutinating antigen was prepared in the fluid phase of suspension cultures. Systems employing baby hamster kidney culture adapted inocula, nonadapted inocula, and cells derived from long-term infected suspension cultures were evaluated. Optimal specific hemagglutinating titers were obtained when kaolin-treated fetal bovine serum was used in the media and when the incoulum had previously been adapted in a suspension culture system. When cultures derived from long-term infected suspension systems were studied, the number of daily harvests in which acceptable titers were present was prolonged. However, titers were generally higher in suspension systems employing cells which had recently been infected. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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