Interplay between nitric oxide and gonadotrophin-releasing hormone in the neuromodulation of the corpus luteum during late pregnancy in the rat
| Autor: | Sandra Vallcaneras, Laura Morales, María Belén Delsouc, Darío Ramirez, Verónica Filippa, Marina Fernández, Carlos M. Telleria, Marilina Casais |
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| Rok vydání: | 2021 |
| Předmět: |
endocrine system
QH471-489 Gestational Age Nervous System Gonadotropin-Releasing Hormone Corpus luteum Endocrinology Pregnancy Neural Pathways Animals Drug Interactions Ganglia Sympathetic End of pregnancy Reproduction Research Ovary Obstetrics and Gynecology Nitric oxide Gynecology and obstetrics Rats Reproductive Medicine Gonadotrophin-releasing hormone RG1-991 Rat Female Ex-vivo coeliac ganglion-superior ovarian nerve-ovary system hormones hormone substitutes and hormone antagonists Developmental Biology |
| Zdroj: | Reproductive Biology and Endocrinology : RB&E Reproductive Biology and Endocrinology, Vol 20, Iss 1, Pp 1-12 (2022) |
| ISSN: | 1477-7827 |
| Popis: | Background Nitric oxide and GnRH are biological factors that participate in the regulation of reproductive functions. To our knowledge, there are no studies that link NO and GnRH in the sympathetic ganglia. Thus, the aim of the present work was to investigate the influence of NO on GnRH release from the coeliac ganglion and its effect on luteal regression at the end of pregnancy in the rat. Methods The ex vivo system composed by the coeliac ganglion, the superior ovarian nerve, and the ovary of rats on day 21 of pregnancy was incubated for 180 min with the addition, into the ganglionic compartment, of L-NG-nitro arginine methyl ester (L-NAME), a non-selective NO synthase inhibitor. The control group consisted in untreated organ systems. Results The addition of L-NAME in the coeliac ganglion compartment decreased NO as well as GnRH release from the coeliac ganglion. In the ovarian compartment, and with respect to the control group, we observed a reduced release of GnRH, NO, and noradrenaline, but an increased production of progesterone, estradiol, and expression of their limiting biosynthetic enzymes, 3β-HSD and P450 aromatase, respectively. The inhibition of NO production by L-NAME in the coeliac ganglion compartment also reduced luteal apoptosis, lipid peroxidation, and nitrotyrosine, whereas it increased the total antioxidant capacity within the corpora lutea. Conclusion Collectively, the results indicate that NO production by the coeliac ganglion modulates the physiology of the ovary and luteal regression during late pregnancy in rats. |
| Databáze: | OpenAIRE |
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