Budding yeast RSI1/APC2, a novel gene necessary for initiation of anaphase, encodes an APC subunit
| Autor: | Kate M. Kramer, Anthony L. Johnson, Didier Fesquet, Leland H. Johnston |
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| Rok vydání: | 1998 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Ubiquitin-Protein Ligases Genes Fungal Molecular Sequence Data Mutant Cell Cycle Proteins Saccharomyces cerevisiae Cyclin B Apc8 Subunit Anaphase-Promoting Complex-Cyclosome Anaphase-Promoting Complex-Cyclosome General Biochemistry Genetics and Molecular Biology Apc2 Subunit Anaphase-Promoting Complex-Cyclosome Fungal Proteins Ligases Cyclins Amino Acid Sequence Kinase activity Molecular Biology Metaphase Cyclin-Dependent Kinase Inhibitor Proteins Anaphase General Immunology and Microbiology biology General Neuroscience Temperature Nuclear Proteins Ubiquitin-Protein Ligase Complexes Sic1 Molecular biology Cell biology Securin Phenotype Mutagenesis Mitotic exit biology.protein Genes Lethal Anaphase-promoting complex Research Article |
| Zdroj: | The EMBO Journal. 17:498-506 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/17.2.498 |
| Popis: | SIC1 is a non‐essential gene encoding a CDK inhibitor of Cdc28‐Clb kinase activity. Sic1p is involved in both mitotic exit and the timing of DNA synthesis. To identify other genes involved in controlling Clb‐kinase activity, we have undertaken a genetic screen for mutations which render SIC1 essential. Here we describe a gene we have identified by this means, RSI1/APC2 . Temperature‐sensitive rsi1/apc2 mutants arrest in metaphase and are unable to degrade Clb2p, suggesting that Rsi1p/Apc2p is associated with the anaphase promoting complex (APC). This is an E3 ubiquitin‐ligase that controls anaphase initiation through degradation of Pds1p and mitotic exit via degradation of Clb cyclins. Indeed, the anaphase block in rsi1/apc2 temperature‐sensitive mutants is overcome by removal of PDS1 , consistent with Rsi1p/Apc2p being part of the APC. In addition, like our rsi1/apc2 mutations, cdc23‐1 , encoding a known APC subunit, is also lethal with sic1Δ . Thus SIC1 clearly becomes essential when APC function is compromised. Finally, we find that Rsi1p/Apc2p co‐immunoprecipitates with Cdc23p. Taken together, our results suggest that RSI1/APC2 is a subunit of APC. |
| Databáze: | OpenAIRE |
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