Modulation of yeast alkaline cation tolerance by Ypi1 requires calcineurin
| Autor: | Ivan Muñoz, José Ramos, Samuel Gelis, Joaquín Ariño, Maribel Marquina, Asier González, Lina Barreto, Mari Carmen Álvarez, Amparo Ruiz |
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| Rok vydání: | 2012 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Protein subunit Phosphatase Saccharomyces cerevisiae Genes Fungal Biology Investigations Gene Expression Regulation Enzymologic Enzyme activator chemistry.chemical_compound Gene Expression Regulation Fungal Cation homeostasis Genetics Homeostasis Cation Transport Proteins Calcineurin Intracellular Signaling Peptides and Proteins biology.organism_classification Adaptation Physiological Phosphoric Monoester Hydrolases Transport protein Enzyme Activation Phenotype Biochemistry chemistry Lithium chloride Sodium-Potassium-Exchanging ATPase Lithium Chloride Plasmids |
| Zdroj: | Genetics. 190(4) |
| ISSN: | 1943-2631 |
| Popis: | Ypi1 was discovered as an essential protein able to act as a regulatory subunit of the Saccharomyces cerevisiae type 1 protein phosphatase Glc7 and play a key role in mitosis. We show here that partial depletion of Ypi1 causes lithium sensitivity and that high levels of this protein confer a lithium-tolerant phenotype to yeast cells. Remarkably, this phenotype was independent of the role of Ypi1 as a Glc7 regulatory subunit. Lithium tolerance in cells overexpressing Ypi1 was caused by a combination of increased efflux of lithium, mediated by augmented expression of the alkaline cation ATPase ENA1, and decreased lithium influx through the Trk1,2 high-affinity potassium transporters. Deletion of CNB1, encoding the regulatory subunit of the calcineurin phosphatase, blocked Ypi1-induced expression of ENA1, normalized Li+ fluxes, and abolished the Li+ hypertolerant phenotype of Ypi1-overexpressing cells. These results point to a complex role of Ypi1 on the regulation of cation homeostasis, largely mediated by the calcineurin phosphatase. |
| Databáze: | OpenAIRE |
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