Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure
| Autor: | Valeria Elisa Perotti, Daniel Eduardo Vazquez, Mariángeles Cocco, Catalina Margarita Anderson, Florencio E. Podestá, Alejandra Soledad Moreno, Fernando Bello, Karina Eva Josefina Tripodi, Guillermo Enrique Meier |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Proteomics
Propanols Physiology GLYCOLYSIS Carbohydrates Carboxylic Acids Plant Science Biology METABOLOMICS Valencia orange Ciencias Biológicas food CITRUS Gene Expression Regulation Plant Freezing Botany Genetics Metabolomics RNA Messenger Cold stress Plant Proteins Flavonoids Cell Biology General Medicine Bioquímica y Biología Molecular food.food Metabolomic profiling RNA Plant Alcohols Fruit PROTEOMICS Carbohydrate Metabolism CIENCIAS NATURALES Y EXACTAS Citrus sinensis |
| Zdroj: | Physiologia Plantarum. 153:337-354 |
| ISSN: | 0031-9317 |
| DOI: | 10.1111/ppl.12259 |
| Popis: | The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage. Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina Fil: Moreno, Alejandra S.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina Fil: Tripodi, Karina Eva Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina Fil: Meier, Guillermo. Estacion Extal. Agrop. Concordia; Argentina Fil: Bello, Fernando. Estacion Extal. Agrop. Concordia; Argentina Fil: Cocco, Mariángeles. Estacion Extal. Agrop. Concordia; Argentina Fil: Vázquez, Daniel. Estacion Extal. Agrop. Concordia; Argentina Fil: Anderson, Catalina. Estacion Extal. Agrop. Concordia; Argentina Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (i); Argentina |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text