Eosinophils in the blood of hematopoietic stem cell transplanted patients are activated and have different molecular marker profiles in acute and chronic graft-versus-host disease

Autor:	Marianne Johnsson, Christine Wennerås, Krista Vaht, Jan-Erik Johansson, Julia Cromvik
Rok vydání:	2014
Předmět:	Pathology medicine.medical_specialty medicine.medical_treatment Immunology CCR3 CD11c chemical and pharmacologic phenomena Hematopoietic stem cell transplantation Chemokine receptor immune system diseases medicine graft-versus-host disease Immunology and Allergy Corticosteroids Original Research Cluster of differentiation business.industry flow cytometry Hematopoietic stem cell Eosinophil respiratory system medicine.disease medicine.anatomical_structure Graft-versus-host disease surgical procedures operative hematopoietic stem cell transplantation eosinophils business
Zdroj:	Immunity, Inflammation and Disease
ISSN:	2050-4527
Popis:	While increased numbers of eosinophils may be detected in patients with graft-versus-host disease (GVHD) following hematopoietic stem cell transplantation, it is not known if eosinophils play a role in GVHD. The aims of this study were to determine: whether eosinophils are activated during GVHD; whether the patterns of activation are similar in acute and chronic GVHD; and the ways in which systemic corticosteroids affect eosinophils. Transplanted patients (n = 35) were investigated for eosinophil numbers and the expression levels of 16 eosinophilic cell surface markers using flow cytometry; all the eosinophil data were analyzed by the multivariate method OPLS-DA. Different patterns of molecule expression were observed on the eosinophils from patients with acute, chronic, and no GVHD, respectively. The molecules that provided the best discrimination between acute and chronic GVHD were: the activation marker CD9; adhesion molecules CD11c and CD18; chemokine receptor CCR3; and prostaglandin receptor CRTH2. Patients with acute or chronic GVHD who received systemic corticosteroid treatment showed down-regulation of the cell surface markers on their eosinophils, whereas corticosteroid treatment had no effect on the eosinophil phenotype in the patients without GVHD. In summary, eosinophils are activated in GVHD, display different activation profiles in acute and chronic GVHD, and are highly responsive to systemic corticosteroids.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d8fa48b0d1ddee501fddc1be45cee654 https://pubmed.ncbi.nlm.nih.gov/25400930 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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