An LC-MS Method for Analyzing Total Resveratrol in Grape Juice, Cranberry Juice, and in Wine
| Autor: | Richard B. van Breemen, Yanan Yang, Yan Wang, Robin Roderick, Florentina Catana |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Light
endocrine system diseases Wine Atmospheric-pressure chemical ionization Resveratrol Mass spectrometry High-performance liquid chromatography Antioxidants Mass Spectrometry Beverages chemistry.chemical_compound Liquid chromatography–mass spectrometry Stilbenes Vitis Selected ion monitoring Piceid Chromatography Chemistry organic chemicals Reproducibility of Results food and beverages General Chemistry Fruit General Agricultural and Biological Sciences Chromatography Liquid |
| Zdroj: | Journal of Agricultural and Food Chemistry. 50:431-435 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | Resveratrol is an antioxidant found in grapes, grape products, and some other botanical sources with antiinflammatory and anticancer properties. In grapes and wine, it occurs both as free resveratrol and piceid, the 3beta-glucoside of resveratrol. Here we report a liquid chromatography-mass spectrometry method to analyze total resveratrol (including free resveratrol and resveratrol from piceid) in fruit products and wine. Samples were extracted using methanol, enzymatically hydrolyzed, and analyzed using reversed phase HPLC with positive ion atmospheric pressure chemical ionization (APCI) mass spectrometric detection. Following APCI, the abundance of protonated molecules was recorded using selected ion monitoring (SIM) of m/z 229. An external standard curve was used for quantitation, which showed a linear range of 0.52-2260 pmol of trans-resveratrol injected on-column with a correlation coefficient 0.9999. The coefficient of variance of the response factor over the same concentration range was determined to be 5.8%, and the intra-assay coefficient of variance was determined to be 4.2% (n = 7). The limit of quantitation, defined as signal-to-noise 10:1, was determined to be 0.31 pmol injected on-column. The extraction efficiency of the method was determined to be 92%. The stability of resveratrol under different conditions was also examined. For example, resveratrol was stable for up to 5 days at 4 degrees C in the dark but was not stable at room temperature without protection from light. Resveratrol was detected in grape, cranberry, and wine samples. Concentrations ranged from 1.56 to 1042 nmol/g in Concord grape products, and from 8.63 to 24.84 micromol/L in Italian red wine. The concentrations of resveratrol were silmilar in cranberry and grape juice at 1.07 and 1.56 nmol/g, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|