Molecular analysis of a sunflower gene encoding an homologous of the B subunit of a CAAT binding factor
| Autor: | Chiara Pucciariello, Claudio Pugliesi, Marco Fambrini, Laura Pistelli, Emanuela Sani, Mariangela Salvini |
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| Přispěvatelé: | Salvini, Mariangela, Sani, E, Fambrini, M, Pistelli, L, Pucciariello, C, Claudio Pugliesi, C. |
| Rok vydání: | 2012 |
| Předmět: |
Helianthus annuus L
Molecular Sequence Data Response element Biology Epigenesis Genetic Epigenetics of physical exercise Gene Expression Regulation Plant Genetics Coding region Amino Acid Sequence Regulatory Elements Transcriptional Molecular Biology Gene Phylogeny Plant Proteins Homeodomain Proteins Regulation of gene expression Binding Sites Base Sequence Sequence Homology Amino Acid Nucleic acid sequence Promoter Sequence Analysis DNA General Medicine DNA Methylation Introns CCAAT binding transcription factor Protein Subunits CCAAT-Binding Factor DNA methylation Helianthus Sequence Alignment epigenetic |
| Zdroj: | Molecular Biology Reports. 39:6449-6465 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-012-1463-9 |
| Popis: | A genomic DNA fragment containing the complete LEAFY COTYLEDON1-LIKE (HaL1L) gene was retrieved by chromosome walking. Its sequence was confirmed and elongated by screening a sunflower genomic DNA BAC Library. HaL1L, whose cDNA had already been sequenced and characterized, encodes a NF-YB subunit of a CCAAT box-binding factor (NF-Y) involved in the early stages of zygotic and somatic embryogenesis in the Helianthus genus. In the HaL1L 50-flanking region, elements specific to a putative TATA-box promoter and two ‘‘CG isles’’ were identified. An investigation of the methylation status of these CG rich DNA regions showed that differentially methylated cytosines were recognizable in the DNA of embryos on the fifth day after pollination in comparison to leaf DNA suggesting that during plant development epigenetic regulation of HaL1L transcription was achieved by methylating cytosine residues. We also searched the HaL1L nucleotide sequence for cis-regulatory elements able to interact with other transcription factors (TFs) involved in the HaL1L regulation. Of the elements identified, one of the most intriguing is WUSATA, the target sequence for the WUSCHEL (WUS) TF, which may be part of a complex regulation network controlling embryo development. In this article, we show that the WUSATA target site, located in the intron of HaL1L, is able to bind the TF WUS. Interestingly, we found auxin and abscisic acid responsive motifs in the HaL1L promoter region suggesting that this gene may additionally by under hormonal control. Finally, the presence of a cytoplasmic polyadenylation signal downstream to the coding region indicates that this gene may also be controlled at the translation level by a temporarily making the pre-synthesized HaL1L mRNA unavailable for protein synthesis. |
| Databáze: | OpenAIRE |
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