Distinct and stage-specific contributions of TET1 and TET2 to stepwise cytosine oxidation in the transition from naive to primed pluripotency

Autor:	Christopher B. Mulholland, Thomas Carell, Sebastian Bultmann, Franziska R. Traube, Edris Parsa, Miha Modic, Joel Ryan, Eva-Maria Eckl, Michael D. Bartoschek, Paul Stolz, Maximillian Schoenung, Heinrich Leonhardt
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	chemistry.chemical_classification chemistry.chemical_compound DNA demethylation Enzyme chemistry Transition (genetics) In vivo Epigenetics Stage specific Cytosine Cell biology
DOI:	10.1101/281519
Popis:	The TET-oxidized cytosine derivatives, 5-hydroxymethylcytosine (5hmC) and 5-formylcytosine (5fC), are considered DNA demethylation intermediates as well as stable epigenetic marks in mammals. We compared modified cytosine and enzyme levels in TET-knockout cells during naive pluripotency exit and found distinct and differentiation-dependent contributions of TET1 and TET2 to 5hmC and 5fC formation. The divergent modified cytosine levels argue for independent consecutive oxidation steps in vivo with broad implications for epigenetic regulation.
Databáze:	OpenAIRE
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