Perinatal exposure to octabromodiphenyl ether mixture, DE-79, alters the vasopressinergic system in adult rats
Autor: | Martha León-Olea, María Isabel Amaya, Patricia de Gortari, Samuel Mucio-Ramírez, Eduardo Sánchez-Islas, Prasada Rao S. Kodavanti, Mhar Y. Alvarez-Gonzalez |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty Vasopressin Offspring Vasopressins Hypothalamus Stimulation Nitric Oxide Synthase Type I Toxicology Nitric Oxide Article Nitric oxide 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Polybrominated diphenyl ethers Osmoregulation Osmotic Pressure Pregnancy Internal medicine medicine Halogenated Diphenyl Ethers Endocrine system Animals Rats Wistar Pharmacology Perinatal Exposure Rats 030104 developmental biology Endocrinology chemistry Animals Newborn Hypothalamus Anterior 030220 oncology & carcinogenesis Environmental Pollutants Female Paraventricular Hypothalamic Nucleus |
Zdroj: | Toxicol Appl Pharmacol |
Popis: | Polybrominated diphenyl ethers (PBDEs) are persistent environmental pollutants considered as neurotoxicants and endocrine disruptors with important biological effects ranging from alterations in growth, reproduction, and effects on the hypothalamus-pituitary-adrenal axis. The vasopressinergic (AVPergic) system is a known target for pentaBDEs mixture (DE-71) and the structurally similar chemicals, polychlorinated biphenyls. However, the potential adverse effects of mixtures containing octaBDE compounds, like DE-79, on the AVPergic system are still unknown. The present study aims to examine the effects of perinatal DE-79 exposure on the AVPergic system. Dams were dosed from gestational day 6 to postnatal day 21 at doses of 0 (control), 1.7 (low) or 10.2 (high) mg/kg/day, and male offspring from all doses at 3-months-old were subjected to normosmotic and hyperosmotic challenge. Male offspring where later assessed for alterations in osmoregulation (i.e. serum osmolality and systemic vasopressin release), and both vasopressin immunoreactivity (AVP-IR) and gene expression in the hypothalamic paraventricular and supraoptic nuclei. Additionally, to elucidate a possible mechanism for the effects of DE-79 on the AVPergic system, both neuronal nitric oxide synthase immunoreactivity (nNOS-IR) and mRNA expression were investigated in the same hypothalamic nuclei. The results showed that perinatal DE-79 exposure AVP-IR, mRNA expression and systemic release in adulthood under normosmotic conditions and more evidently under hyperosmotic stimulation. nNOS-IR and mRNA expression were also affected in the same nuclei. Since NO is an AVP regulator, we propose that disturbances in NO could be a mechanism underlying the AVPergic system disruption following perinatal DE-79 exposure leading to osmoregulation deficits. |
Databáze: | OpenAIRE |
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