Different frequencies of Tc17/Tc1 and Th17/Th1 cells in chronic spontaneous urticaria
| Autor: | António Martinho, Susana Pedreiro, Ana Lopes, D Machado, Artur Paiva, Beatriz Tavares, Celso Pereira, Ana Henriques, Isabel Silva, C Chieira, Maria de Jesus Inácio, Maria Luísa Pais |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Urticaria Immunology chemical and pharmacologic phenomena medicine.disease_cause Real-Time Polymerase Chain Reaction Flow cytometry Text mining immune system diseases parasitic diseases medicine Immunology and Allergy Humans RNA Messenger skin and connective tissue diseases Chronic urticaria medicine.diagnostic_test business.industry RNA General Medicine biochemical phenomena metabolism and nutrition Immune dysregulation Middle Aged Th1 Cells Flow Cytometry Real-time polymerase chain reaction Leukocytes Mononuclear Cytokines Th17 Cells Female business T-Lymphocytes Cytotoxic |
| Zdroj: | International archives of allergy and immunology. 161(2) |
| ISSN: | 1423-0097 |
| Popis: | Background: Chronic urticaria is associated with an immune dysregulation usually mediated by T lymphocytes. Recently, Th17 and Tc17 have been implicated in autoimmune diseases; however, their role in urticaria is not clear yet. Methods: For the study we recruited 20 patients [10 of them had autoreactive chronic spontaneous urticaria (positive autologous intradermal serum test response, ASST+), and the other 10 were nonautoreactive chronic spontaneous urticaria patients (ASST–)] and 17 healthy age- and gender-matched controls (HG). The frequency and functional activity of Th17/Tc17 and Th1/Tc1 cells were evaluated by flow cytometry and type 2 cytokine mRNA by real-time PCR. Results: Our results demonstrated a significant decrease in Th17 frequency in both chronic urticaria groups compared to HG; regarding the amount of IL-17, at the single cell level, it was reduced in ASST– compared to HG. Concerning the Th1 and Th17 cells producing IFN-γ, IL-2, and TNF-α, a lower frequency was noted in chronic urticaria patients compared to HG. In contrast, a significantly increased frequency of Tc1 cells producing these cytokines was noted in ASST+ compared to HG and ASST–. Also, the frequency of Tc17 cells producing TNF-α was increased in ASST+ compared to HG; however, with respect to the amount of TNF-α, at the single cell level, we found a decrease in ASST+ compared to HG. Regarding type 2 cytokine mRNA, a higher expression was verified in ASST+ compared to HG. Conclusion: Our data suggest a probable involvement of cytotoxic T cells, mainly the Tc1 and Tc17 subsets, in chronic urticaria, particularly in the ASST+ group. |
| Databáze: | OpenAIRE |
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