Direct measurement of a tethered ligand-receptor interaction potential
| Autor: | Tonya L. Kuhl, Joyce Wong, Jacob N. Israelachvili, Samuel Zalipsky, Nasreen Mullah |
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| Rok vydání: | 1997 |
| Předmět: |
Streptavidin
Multidisciplinary Binding Sites Chemical Phenomena Stereochemistry Chemistry Physical Lipid Bilayers Molecular Conformation Receptor interaction Biotin Surface forces apparatus Ligand (biochemistry) Ligands Polyethylene Glycols chemistry.chemical_compound Interaction potential chemistry Bacterial Proteins Models Chemical Biophysics Binding site Lipid bilayer Receptor Mathematics |
| Zdroj: | Science (New York, N.Y.). 275(5301) |
| ISSN: | 0036-8075 |
| Popis: | Many biological recognition interactions involve ligands and receptors that are tethered rather than rigidly bound on a cell surface. A surface forces apparatus was used to directly measure the force-distance interaction between a polymer-tethered ligand and its receptor. At separations near the fully extended tether length, the ligands rapidly lock onto their binding sites, pulling the ligand and receptor together. The measured interaction potential and its dynamics can be modeled with standard theories of polymer and colloidal interactions. |
| Databáze: | OpenAIRE |
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