N-Acetyl Glucosamine Obtained from Chitin by Chitin Degrading Factors in Chitinbacter tainanesis
| Autor: | Bing-Shiun Fang, Chao-Hsien Yeh, Chao-Lin Liu, Tung-Li Huang, Chia-Rui Shen, Jeen-Kuan Chen |
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| Rok vydání: | 2011 |
| Předmět: |
macromolecular substances
Chitinibacter tainanensis chitin Article Catalysis Acetylglucosamine lcsh:Chemistry Inorganic Chemistry chemistry.chemical_compound Bacterial Proteins Chitin Glucosamine Acetylglucosaminidase N acetyl glucosamine Physical and Theoretical Chemistry fermentation lcsh:QH301-705.5 Molecular Biology Spectroscopy chemistry.chemical_classification biology Chitinases CDFs fungi Organic Chemistry NAG Betaproteobacteria General Medicine Computer Science Applications carbohydrates (lipids) Enzyme lcsh:Biology (General) lcsh:QD1-999 chemistry Biochemistry Chitinase biology.protein Fermentation |
| Zdroj: | International Journal of Molecular Sciences, Vol 12, Iss 2, Pp 1187-1195 (2011) International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 12; Issue 2; Pages: 1187-1195 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms12021187 |
| Popis: | A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation. |
| Databáze: | OpenAIRE |
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