Maintenance and Acceleration of Pericellular Matrix Formation within 3D Cartilage Cell Culture Models
| Autor: | Ying Yang, H A Owida, Nicola L Kuiper |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cartilage
Articular 0301 basic medicine Acceleration Cell Culture Techniques Biomedical Engineering Physical Therapy Sports Therapy and Rehabilitation Articular cartilage Collagen Type VI 02 engineering and technology Matrix (biology) Chondrocyte 03 medical and health sciences medicine Immunology and Allergy Clinical Research papers Chemistry Cartilage Mesenchymal stem cell 021001 nanoscience & nanotechnology Extracellular Matrix Cell biology 030104 developmental biology medicine.anatomical_structure Cell culture Type VI collagen 0210 nano-technology |
| Zdroj: | Cartilage |
| ISSN: | 1947-6043 1947-6035 |
| Popis: | Objective In native articular cartilage, chondrocytes are surrounded by a thin pericellular matrix (PCM) forming chondrons. The PCM is exclusively rich in type VI collagen. The retention of the PCM has a significant influence on the metabolic activity of the chondrocytes. Design This study investigated the influence of 2 hydrogels (hyaluronic acid [HA] and agarose) and 2 media compositions (basal and chondrogenic) on the preservation/maintenance and acceleration of PCM formation over a 21-day time course. Different combinations of chondrocytes, chondrons, and mesenchymal stem cells (MSCs) were studied. Results Both hydrogels preserved chondrons PCM from day 1 up to 21-day culture regardless of media composition. Type VI collagen immunostaining of the cultured chondrons appeared both dense and homogenous. The presence of MSCs did not influence this outcome. At day 1, type VI collagen was not present around chondrocytes alone or their co-culture with MSCs. In the HA hydrogel, type VI collagen was located within the PCM after 7 days in both mono- and co-cultures. In the agarose hydrogel, collagen VI was located within the PCM at 7 days (co-cultures) and 14 days (monocultures). In both hydrogel systems, chondrogenic media enhanced the production of key extracellular matrix components in both mono- and co-cultures in comparison to basal media (11.5% and 14% more in glycosaminoglycans and type II collagen for chondrocytes samples at day 21 culture samples, respectively). However, the media types did not enhance type VI collagen synthesis. Conclusion Altogether, a 3D chondrogenic hydrogel environment is the primary condition for maintenance and acceleration of PCM formation. |
| Databáze: | OpenAIRE |
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