FLOT1 promotes tumor development, induces epithelial–mesenchymal transition, and modulates the cell cycle by regulating the Erk/Akt signaling pathway in lung adenocarcinoma

Autor: Yuan Mao, Lin Xu, Jun Wang, Weifei Fan, Qixing Mao, Yan Chen, Li Xu, Louqian Zhang
Rok vydání: 2019
Předmět:
signaling pathway
0301 basic medicine
Pulmonary and Respiratory Medicine
MAPK/ERK pathway
Scaffold protein
Epithelial-Mesenchymal Transition
Lung Neoplasms
MAP Kinase Signaling System
Adenocarcinoma of Lung
lcsh:RC254-282
FLOT1
03 medical and health sciences
malignant behavior
0302 clinical medicine
Humans
Medicine
Epithelial–mesenchymal transition
Cell Proliferation
Cell growth
business.industry
Akt/PKB signaling pathway
Cell Cycle
Membrane Proteins
Original Articles
General Medicine
Cell cycle
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
lung adenocarcinoma
Cell biology
Gene Expression Regulation
Neoplastic
030104 developmental biology
Oncology
A549 Cells
Apoptosis
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Original Article
Signal transduction
business
Proto-Oncogene Proteins c-akt
Signal Transduction
Zdroj: Thoracic Cancer
Thoracic Cancer, Vol 10, Iss 4, Pp 909-917 (2019)
ISSN: 1759-7714
1759-7706
DOI: 10.1111/1759-7714.13027
Popis: Background FLOT1 is a scaffolding protein of lipid rafts that is believed to be involved in numerous cellular processes. However, few studies have explored the function of FLOT1 in the development of lung adenocarcinoma (LUAD) and the underlying mechanisms of FLOT1 activity. Methods FLOT1 knockdown and overexpression models were constructed via lentivirus. Cell growth, invasion, migration, and apoptosis were detected to evaluate the role of FLOT1 in LUAD development. Epithelial–mesenchymal transition (EMT) and cell cycle regulatory markers were then examined. Finally, the influence of FLOT1 on the Erk/Akt signaling pathway was investigated. Results FLOT1 promoted cell growth, invasion, and migration and inhibited cell apoptosis. In addition, FLOT1 induced EMT and modulated the cell cycle by activating the Erk/Akt signaling pathway. Conclusion The findings indicate a significant role of FLOT1 in LUAD development. Targeting FLOT1 may be a potential therapeutic strategy for LUAD.
Databáze: OpenAIRE
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