Germination Induces Accumulation of Specific Proteins and Antifungal Activities in Corn Kernels
| Autor: | Robert L. Brown, Alan R. Lax, Zhi-Yuan Chen, Thomas E. Cleveland, Neil W. Widstrom, A. D. Mehta, Baozhu Guo, J. S. Russin, C. P. Selitrennikoff |
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| Rok vydání: | 1997 |
| Předmět: |
Gel electrophoresis
Aflatoxin food.ingredient genetic structures medicine.diagnostic_test biology food and beverages Aspergillus flavus Plant Science Fungi imperfecti biology.organism_classification Corn kernel food Western blot Biochemistry Germination Botany medicine Imbibition Agronomy and Crop Science |
| Zdroj: | Phytopathology®. 87:1174-1178 |
| ISSN: | 1943-7684 0031-949X |
| DOI: | 10.1094/phyto.1997.87.11.1174 |
| Popis: | Guo, B. Z., Chen, Z.-Y., Brown, R. L., Lax, A. R., Cleveland, T. E., Russin, J. S., Mehta, A. D., Selitrennikoff, C. P., and Widstrom, N. W. 1997. Germination induces accumulation of specific proteins and antif ungal activities in corn kernels. Phytopathology 87:1174-1178. This study examined protein induction and accumulation during imbibition and germination of corn kernels, as well as antifungal activ ities of extracts from germinating kernels against Aspergillus flavus and Fusarium moniliforme. Genotypes studied included GT-MAS:gk and Mp420, which are resistant to A. flavus infection and aflatoxin accumulation, and Pioneer 3154 and Deltapine G-4666, which are susceptible to A. flavus infection and aflatoxin accumulation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis resolved five protein bands that were present at higher concentrations in germinated kernels than in nongerminated kernels. Western blot analyses revealed that one of these proteins reacted with the 22-kDa zeamatin antiserum, and a zeamatin-like protein accumulated to a higher concentration in germinated kernels. Two protein bands from dry kernels that reacted with ribosome-inactivating protein (RIP) antiserum were identified as the 32-kDa proRIP-like form and an 18-kDa peptide of the two peptides that form active RIP. However, in germinated kernels, two protein bands that reacted with RIP antiserum were identified as two RIP-like peptides with a molecular mass of ≈18 and 9 kDa. Purified RIP and zeamatin from corn inhibited growth of A. flavus. Bioassays of germinated kernel extracts from all four genotypes exhibited antifungal activity against A. flavus and F. moniliforme, with extracts from the susceptible genotypes showing greater inhibition zones. This study provides evidence of protein induction in corn kernels during imbibition or the early stages of germination, and the induced proteins may be related to our previous findings of germination-associated resistance in the corn kernel, especially in the susceptible kernels. |
| Databáze: | OpenAIRE |
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