Amplicon sequencing reveals the bacterial diversity in milk, dairy premises and Serra da Canastra artisanal cheeses produced by three different farms
| Autor: | Anderson S. Sant'Ana, Rafaela C. Baptista, Melline F. Noronha, Bruna A. Kamimura, Henry M. Nascimento, Lucélia Cabral |
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| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Farms biology Bacteria 030306 microbiology Microbial diversity Lactococcus Microbiota Sequencing data Dairy milk Raw milk biology.organism_classification Microbiology 03 medical and health sciences Starter Milk Cheese RNA Ribosomal 16S Amplicon sequencing Food Microbiology Animals Food science Relative species abundance 030304 developmental biology Food Science |
| Zdroj: | Food microbiology. 89 |
| ISSN: | 1095-9998 |
| Popis: | In this work, the amplicon sequencing of the 16 S rRNA gene was employed to investigate the bacterial diversity in ingredients, processing environment, and ripened cheeses collected from three farms producing Serra da Canastra artisanal cheese. The data obtained indicated a remarkable variability in the bacteria consortia of the milk, whey, and environmental samples collected in farms 1, 2, and 3, despite their location in the same city. On the other hand, the starter culture and final product (ripened cheese) presented more constant and similar microbiota no matter the farm. The findings suggest that Streptococcus and Lactococcus have competitive advantages throughout Serra da Canastra cheese-making/ripening, which is crucial for their high relative abundance in the final products. An exploratory assessment based on sequencing data available in the literature showed that the Serra da Canastra cheeses sequences clustered with specific cheese varieties that are also made from raw milk but ripened for very different periods. The findings of this study highlight that despite the variability of milk and whey microbiota among the three farms, the starter culture (“pingo”) has strong relevance in shaping the microbiota of the final product. |
| Databáze: | OpenAIRE |
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