The activity of the saponin ginsenoside Rh2 is enhanced by the interaction with membrane sphingomyelin but depressed by cholesterol
| Autor: | Laurence Lins, Magali Deleu, Maria Janikowska-Sagan, Donatienne Tyteca, Emily J. S. Claereboudt, Sandrine L. Verstraeten, Marie-Paule Mingeot-Leclercq |
|---|---|
| Přispěvatelé: | UCL - SSS/LDRI - Louvain Drug Research Institute, UCL - SSS/DDUV/CELL - Biologie cellulaire |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell Membrane Permeability Ginsenosides Membrane Fluidity Panax lcsh:Medicine Article 03 medical and health sciences chemistry.chemical_compound Membrane biophysics 0302 clinical medicine Phosphatidylcholine Membrane activity Membrane fluidity polycyclic compounds Animals lcsh:Science Unilamellar Liposomes Multidisciplinary Molecular medicine Cholesterol Vesicle Egg Proteins lcsh:R technology industry and agriculture Sphingomyelins 030104 developmental biology Digitonin Membrane chemistry Biophysics Phosphatidylcholines lipids (amino acids peptides and proteins) lcsh:Q Sphingomyelin Chickens 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019) Scientific Reports Scientific reports, Vol. 9, no.1, p. 7285 [1-14] (2019) |
| ISSN: | 2045-2322 |
| Popis: | The membrane activity of some saponins, such as digitonin or alpha-hederin, is usually attributed to their interaction with membrane cholesterol (Chol). This contrasts with our recent publication showing that Chol, contrary to sphingomyelin (SM), can delay the cytotoxicity of the saponin ginsenoside Rh2, challenging the usual view that most saponins mediate their membrane effects through interaction with Chol. The aim of the present study was to elucidate the respective importance of Chol and SM as compared to phosphatidylcholine (PC) species in the membrane-related effects of Rh2. On simple lipid monolayers, Rh2 interacted more favorably with eggSM and DOPC than with Chol and eggPC. Using Large Unilamellar Vesicles (LUVs) of binary or ternary lipid compositions, we showed that Rh2 increased vesicle size, decreased membrane fluidity and induced membrane permeability with the following preference: eggSM:eggPC > eggSM:eggPC:Chol > eggPC:Chol. On Giant Unilamellar Vesicles (GUVs), we evidenced that Rh2 generated positive curvatures in eggSM-containing GUVs and small buds followed by intra-luminal vesicles in eggSM-free GUVs. Altogether, our data indicate that eggSM promotes and accelerates membrane-related effects induced by Rh2 whereas Chol slows down and depresses these effects. This study reconsiders the theory that Chol is the only responsible for the activity of saponins. |
| Databáze: | OpenAIRE |
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