Changes in Amino Acid Profile in Roots of Glyphosate Resistant and Susceptible Soybean (Glycine max) Induced by Foliar Glyphosate Application
| Autor: | José Manuel Camiña, Carlos Alberto Moldes, Siu Mui Tsai, Miguel Angel Cantarelli, Ricardo Antunes Azevedo |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine AMINO ACID PROFILE Otras Ciencias Biológicas Transgene Glycine chemistry.chemical_element GLYPHOSATE Biology Plant Roots 01 natural sciences High-performance liquid chromatography SOYBEAN Ciencias Biológicas 03 medical and health sciences chemistry.chemical_compound Amino Acids Chromatography High Pressure Liquid chemistry.chemical_classification Herbicides MULTIVARIATE ANALYSIS food and beverages General Chemistry Metabolism Nitrogen Amino acid 030104 developmental biology chemistry Biochemistry Glyphosate TRANSGENIC Soybeans General Agricultural and Biological Sciences CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 65:8823-8828 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.7b03676 |
| Popis: | Amino acid profiles are useful to analyze the responses to glyphosate in susceptible and resistant soybean lines. Comparisons of profiles for 10 amino acids (Asp, Asn, Glu, Gln, Ser, His, Gly, Thr, Tyr, Leu) by HPLC in soybean roots were performed in two near isogenic pairs (four varieties). Foliar application of glyphosate was made to soybean plants after 5 weeks of seeding. Roots of four varieties were collected at 0 and 72 h after glyphosate application (AGA) for amino acid analysis by HPLC. Univariate analysis showed a significant increase of several amino acids in susceptible as well as resistant soybean lines; however, amino acids from the major pathways of carbon (C) and nitrogen (N) metabolism, such as Asp, Asn, Glu and Gln, and Ser, increased significantly in susceptible varieties at 72 h AGA. Multivariate analysis using principal component analysis (2D PCA and 3D PCA) allowed different groups to be identified and discriminated based on the soybean genetic origin, showing the amino acid responses on susceptible and resistant varieties. Based on the results, it is possible to infer that the increase of Asn, Asp, Glu, Gln, and Ser in susceptible varieties would be related to the deregulation of C and N metabolism, as well as changes in the growth mechanisms regulated by Ser. Fil: Moldes, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Cantarelli, Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Camiña, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Tsai, Siu Mui. Universidade de Sao Paulo; Brasil Fil: Antunez Acevedo, Ricardo. Universidade de Sao Paulo; Brasil |
| Databáze: | OpenAIRE |
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