Label‐free Voltammetric Detection of Products of Terminal Deoxynucleotidyl Transferase Tailing Reaction
| Autor: | Monika Hermanová, Swathi Senthil Kumar, Miroslav Fojta, Richard P. Bowater, Anna Ondráčková, Pavlína Havranová-Vidláková |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Oligonucleotide
010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences Combinatorial chemistry 0104 chemical sciences Analytical Chemistry Nucleobase chemistry.chemical_compound Terminal deoxynucleotidyl transferase chemistry Hanging mercury drop electrode Electrode Molecule 0210 nano-technology terminal deoxynucleotidyl transferase oligonucleotide tailing DNA electrochemistry label free nucleobase reduction oxidation DNA |
| Zdroj: | Electroanalysis |
| ISSN: | 1521-4109 1040-0397 |
| DOI: | 10.1002/elan.201800452 |
| Popis: | A label-free approach that takes advantage of intrinsic electrochemical activity of nucleobases has been applied to study the products of terminal deoxynucleotidyl transferase (TdT) tailing reaction. DNA homooligonucleotides A 30 , C 30 and T 30 were used as primers for the tailing reaction to which a dNTP – or a mixture of dNTPs – and TdT were added to form the tails. Electrochemical detection enabled study of the tailing reaction products created by various combinations of primers and dNTPs, with pyrolytic graphite electrode (PGE) being suitable for remarkably precise analysis of the length of tailing reaction products. Furthermore, the hanging mercury drop electrode (HMDE) was able to reveal formation of various DNA structures, such as DNA hairpins and G-quadruplexes, which influence the behavior of DNA molecules at the negatively charged surface of HMDE. Thus, the described approach proves to be an excellent tool for studying the TdT tailing reactions and for exploring how various DNA structures affect both the tailing reactions and electrochemical behavior of DNA oligonucleotides at electrode surfaces. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |