Preclinical and clinical studies with recombinant human basic fibroblast growth factor
Autor: | F. Bertolero, C. Jacob, G. Mazue, Paolo Sarmientos, R. Roncucci |
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Rok vydání: | 1991 |
Předmět: |
Tympanic Membrane
Basic fibroblast growth factor Pharmacology In Vitro Techniques medicine.disease_cause General Biochemistry Genetics and Molecular Biology Corneal Diseases chemistry.chemical_compound History and Philosophy of Science In vivo Cornea Pleiotropism medicine Animals Humans Cloning Molecular Wound Healing business.industry General Neuroscience Recombinant Proteins Rats medicine.anatomical_structure chemistry Tolerability Toxicity Fibroblast Growth Factor 2 Rabbits Irritation Wound healing business |
Zdroj: | Annals of the New York Academy of Sciences. 638 |
ISSN: | 0077-8923 |
Popis: | Basic fibroblast growth factor is a polypeptide belonging to a family of natural proteins also known as heparin-binding growth factors endowed with a pleiotropism of biological activities, the most striking of which are related to wound healing. Large quantities of recombinant human basic fibroblast growth factor (rh-bFGF) of a clinical grade were obtained and used to undertake preclinical and clinical studies. In vivo the wound healing effect of rh-bFGF was evaluated in experimental targets such as the cornea and the tympanic membrane, showing a significantly increased epithelial healing rate in drug-treated animals. The deposition of labeled rh-bFGF after topical applications in ocular wounding models did not result in a systemic absorption of the intact rh-bFGF molecule. The acute and the subchronic toxicity studies undertaken after iv and topical administration of a stable pharmaceutical formulation of rh-bFGF did not result in irritation, and no signs of general toxicity were observed. Altogether these data permitted us to start recently with human studies, which are still ongoing, aimed to evaluate the tolerability and the activity of rh-bFGF on tegumental targets such as the cornea and the skin. |
Databáze: | OpenAIRE |
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