Generation of infectious and transmissible virions from a GB virus B full-length consensus clone in tamarins
| Autor: | Amedeo De Tomassi, Cinzia Traboni, Domenico Lazzaro, Andrea Sbardellati, Ernst J. Verschoor, Elisa Scarselli |
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| Rok vydání: | 2001 |
| Předmět: |
Transcription
Genetic Hepatitis C virus Secondary infection Molecular Sequence Data Genome Viral Biology medicine.disease_cause Virus Immune system Antigen Virology medicine Animals Hepatitis Antibodies Gene Hepatitis Base Sequence Flaviviridae Virion RNA medicine.disease Liver Hepatitis Viral Animal Leukocytes Mononuclear RNA Viral Saguinus |
| Zdroj: | The Journal of general virology. 82(Pt 10) |
| ISSN: | 0022-1317 |
| Popis: | The strong similarity between GB virus B (GBV-B) and hepatitis C virus (HCV) makes tamarins infected by GBV-B an acceptable surrogate animal model for HCV infection. Even more attractive, for drug discovery purposes, is the idea of constructing chimeric viruses by inserting HCV genes of interest into a GBV-B genome frame. To accomplish this, infectious cDNA clones of both viruses must be available. The characterization of several HCV molecular clones capable of infecting chimpanzees has been published, whereas only one infectious GBV-B clone inducing hepatitis in tamarins has been reported so far. Here we describe the infection of tamarins by intrahepatic injection of RNA transcribed from a genomic GBV-B clone (FL-3) and transmission of the disease from infected to naive tamarins via serum inoculation. The disease resulting from both direct and secondary infection was characterized for viral RNA titre and hepatitis parameters as well as for viral RNA distribution in the hepatic tissue. Host humoral immune response to GBV-B antigens was also monitored. The progression of the disease was compared to that induced by intravenous injection of different amounts of the non-recombinant virus. |
| Databáze: | OpenAIRE |
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